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Remarkably Responsive Temperature Control Solutions®


Over Years APRIL 2017 - VOLUME 42 ISSUE 3


From PolyScience


For fi ve decades, PolyScience has responded to the needs of laboratory, chemical, medical, industrial, and plastics markets with unparalleled innovation, passion and user-focus. Our dedication to quality, ease of operation and reliability has helped us become a global company that spans six continents.


Our Refrigerated Circulators, Heated Circulators, Chillers, Recirculating Coolers, Water Baths and special-purpose products are designed to simplify tasks, take less space, integrate more seamlessly and perhaps most important, make your work productive and enjoyable.


Engineered and designed in Chicago, USA, all our products are backed by our comprehensive warranty.


For more information, visit www.PolyScience.com or email us at sales@polyscience.com.


7205ad@reply-direct.com 7205ad@reply-direct.com OFC - Polyscience.indd 1 29/03/2017 08:52 Mass Spectrometry & Spectroscopy


Leaf Polyphenols as Indicators of Climatic Adaptation of Beech (Fagus sylvatica L.) – an HPLC-MS/MS via MRM Approach


Tamás Hofmann, Esztella Tálos-Nebehaj and Levente Albert Microscopy & Microtechniques Head Office - International Labmate Ltd


Oak Court, Sandridge Park, St AIbans, Hertfordshire AL3 6PH, United Kingdom. Tel: +44 (0)1727 855574 • Fax: +44 (0)1727 841694 Amend your subscription: subscribe@intlabmate.com Other enquiries: info@intlabmate.comsales@intlabmate.com ISSN 0143-5140


Publishers of: International Labmate, Labmate UK & Ireland, Lab Asia, Chromatography Today, International Environmental Technology, Asian Environmental Technology, Pollution Solutions, Petro Industry News and Measurement Analysis China


JOEL TEM at Diamond’s Electron Physical Sciences Imaging Centre (ePSIC) Steve Pritchard


Features Spotlight


Safety, Hazard Containment & Sterilising Equipment Gywneth Astles


Publisher Michael H. Pattison


Director of Publications Marcus Pattison


michael@intlabmate.com marcus@intlabmate.com


International Sales & Marketing Manager Chris Jarvis


North American Area Manager Carol Hardy


Director, Asia Pacific Marie Izzard


European Sales Manager John Hobbs-Morris


Online Manager Rob Parker


Guide Manager Sam Jackson


Guide Assistant Lisa Penn


Administrator Helen Wills


chris@intlabmate.com carol@intlabmate.com marie@intlabmate.com.au john@intlabmate.com rob@intlabmate.com sam@intlabmate.com lisapenn@intlabmate.com helenw@intlabmate.com


Editor Gwyneth Astles


Contributing Editor Heather Hobbs


gwyneth@intlabmate.com Spotlight heather@intlabmate.com


Contributing Editor - Separation Science Trevor Hopkins


Production Manager Robert Battell


Production Team Caroline Belt Jill Mitchell


Heather Vincent


Finance Controller Chris Brown


Accounts Assistant Maureen Woodhouse


Credit Control Patricia Rogers


Subscriptions Manager Maureen Woodhouse


Market Research trevor@intlabmate.com robert@intlabmate.com Spotlight


caroline@intlabmate.com jill@intlabmate.com


heatherv@intlabmate.com chris@labmate.net maureen@labmate.net patricia@labmate.net


maureen@labmate.net Marstonbury Ltd


News


Research & Events News Heather Hobbs


Published by International Labmate Ltd - Printed by Headley Brothers Ltd


International Labmate is distributed to scientists, technologists, laboratory technicians and environmentalists throughout the Europe and Middle East, free of charge. The publication has the support of leading suppliers of equipment in the region and is displayed at the major exhibitions and conferences in these markets to attract additional readership.


International Labmate is a member of the


following trade association:


Heather Hobbs News & Views


International Labmate, ISSN 0143-5140, is published 7 times a year - in February, March, April, May, July, September and November by International Labmate Ltd, Oak Court, Sandridge Park, St Albans, Hertfordshire, AL3 6PH, United Kingdom. The US annual subscription price is $100 USD.


Airfreight and mailing in the USA by agent named Air Business Ltd, c/o Worldnet Shipping Inc.,156-15, 146th Avenue, 2nd Floor, Jamaica, NY 11434, USA. Periodicals postage paid at Jamaica NY 11431. US Postmaster: Send address changes to International Labmate, Worldnet Shipping Inc., 156-15, 146th Avenue, 2nd Floor, Jamaica, NY 11434, USA Subscription records are maintained at International Labmate Ltd, Oak Court, Sandridge Park, St Albans, Hertfordshire, AL3 6PH, United Kingdom.


Air Business Ltd is acting as our mailing agent.


North American Office: Carol Hardy - International Labmate Ltd 333 Colony Blvd. #194 The Villages, FL 32162, United States Tel: +(1) 623-869-0184 Fax: +(1) 623-321-9200. E-mail: carol@intlabmate.com


Asian Office: Marie Izzard - International Labmate Ltd PO Box 374, Mona Vale, NSW, 1660, Australia Tel: +61 (0) 2 9918 0317 Fax: +61 (0) 2 9918 0316 E-mail: marie@intlabmate.com.au


Japan: Mr Koichi Ogawa - OTO Research Corporation Takeuchi Building 1 -34-12 Takatanobaba, Shinjuku-Ku, Tokyo 169, Japan Tel: (03) 3208 7821 Fax: (03) 3200 2889


Korea: Pearl Yoon-Joo Jin - The Peak Media Co Ltd Suite 1060, 10th Fl., Trapalace Bldg, 234 HwangSaeUl-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea 13595. Tel: +82 (0)70 4639 3008 Fax: +82 (0)31 712 3228 Email: pearl@tpmkr.com


Business Opportunities Heather Hobbs


Microscopy News Heather Hobbs


International Labmate is published six times per annum in tabloid format. In addition a most comprehensive “Guide to Laboratory and Environmental Equipment” printed in A4 format. It incorporates companies, products and dealers, mailed in May/June.


Although every effort is made to ensure the accuracy of material published, International Labmate and its offices cannot accept responsibility for claims made by contributors, manufacturers or advertisers.


© All rights reserved by International Labmate Ltd. Reproduction in part or whole without written permission is strictly prohibited. Please look after the environment by recycling this magazine Correction Note


In the January/February 2017 issue of International Labmate there was an error in an article written by Mott Corporation where they claimed their mixer was ‘providing better mixing effi ciency using less internal fl ow volume than any mixer currently used in the chromatography industry’. This should have read ‘providing better mixing effi ciency using less internal volume than many comparable mixers used in the chromatography industry’. International Labmate apologise for this error.


Heather Hobbs Pittcon News


2


Drug Discovery & Pharmaceuticals Gywneth Astles


48


Biotechnology & Immunology Gywneth Astles


38 28 56 12


In this Issue


Also available as an e-Journal at: labmate-online.com/ejournal/


Articles Chromatography


Capillary Isoelectric Focusing – A Comparison of Different Carrier Ampholytes for Monoclonal Antibody Charge Heterogeneity Analysis Christian Wenz


4


18


20


54


58


1


7


9


7


4


2


0


1


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