Biototechnology & Immunology


Multi-Parameter Flow Cytometer Delivers High Complexity Cellular Analysis


Beckman Coulter Life Sciences continues to expand the multi-parameter capabilities of its patented CytoFLEX flow cytometer technology. Its latest model, the CytoFLEX LX, with up to six lasers and 21 fluorescent channels, delivers even more parameters with the same exceptional fluorescence sensitivity and superior nanoparticle detection demanded by high end researchers.


With the CytoFLEX LX series, the platform’s innovative excitation and detection capabilities have been further leveraged to meet the high-content needs of researchers interested in up to 23 parameters of cellular analytical measurements.


The ease of use and maintenance of CytoFLEX LX now enables many more researchers to use the system and push their science to this high level. Moreover, other labs which typically used shared lab resources for such complex experiments can now consider accelerating their work with their own benchtop system.


The CytoFLEX sets new standards of fluorescence sensitivity by minimising light loss, so that even dim populations can easily be detected. It can measure as low as ten molecules of fluorescence and measure particles as small as 0.2 micrometers in size.


With the CytoFLEX LX, researchers can measure two scatter parameters in addition to up to 19 fluorescent markers with five lasers; and up to 21 fluorescent markers when six lasers are activated. The patent-pending optical design of the system optimises excitation and light collection efficiency from up to six lasers (375, 405, 488, 561, 638, and 808 nm).


A Wavelength Division Multiplexing (WDM) detection module (also patent-pending) features a bandpass-only design that enables various configurations from four to 21 colours, and contains the full complement of repositionable optical filters.


A new version of Beckman Coulter’s CytoFLEX acquisition software, CytExpert Version 2.0, has also been introduced and is available on the new CytoFLEX LX series. By means of a novel compensation library, users are easily able to set up high colour applications, whether they are in the lab or working remotely.


With CytExpert Version 2.0, researchers can compare and reproduce experimental data over time and across multiple systems, confident that they are reporting accurate and standardised results for each specific application. Other features ideal for high throughput labs include microtitre plate-based analytics; as well as the ability to interface with front-end automation systems. The software also provides tools facilitating compliance with 21CFR11 regulations such as user management, audit trails, and electronic signatures.


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New Microinjectors Provide Optimal Sample Control


The new CellTram® 4 Air and CellTram® 4 Oil from leading life science company Eppendorf raise the bar in terms of ease of use and performance for manual microinjectors.


The CellTram® 4 Air is a pneumatic injector ideal for gentle holding of cells or embryos in suspension. At the same time it is highly suitable for the uptake and injection of cells (e.g., of sperm or ES cells). Therefore, users can set up their workstation with air injectors on both the holding and injection side and conduct microinjections entirely oil-free. Additional new features of the CellTram 4 Air are the dual coarse/fine drive, and the piston position scale, which allows setting the injector’s pressure characteristics according to personal preference.


The CellTram® 4 Oil is optimal for users who prefer the direct responsiveness of an oil-filled injector. It features a new oil-filling system that minimises oil spills and helps save time during the filling process.


Both models have been designed with special emphasis on excellent ergonomics, ease of use and high precision. They also feature improved accessories, such as a scaled capillary holder 4 for reproducible mounting, and a new grip head 4 for easier capillary exchange and better user safety. For transgenic customers, a new capillary holder 4 (slim shape) is available that allows for injecting at angles <15° in combination with Eppendorf manipulators. Flat angle injections can help minimize mechanical trauma, thereby contributing to higher survival rates of the injected cells.


In Europe, the CellTram® 4m Air and Oil are registered as medical device (according to Medical Device Directive 93/42/EEC).


Dr Rudolf Walczak, Global Product Manager Cell handling, said: “Eppendorf has served as expert partners to embryologists and microinjectionists for more than 30 years. We are happy to offer our customers the new and highly improved CellTram 4 injectors. We are dedicated to helping them achieve the best possible results, both in research and in clinical applications.” 41916pr@reply-direct.com


Research Products for Investigating Cellular Metabolism


Amsbio offers an extensive range of proteins, antibodies, assays and kits covering every single target of major cellular metabolic pathways including folate metabolism, pyruvate metabolism (with and without oxygen), citrate metabolism, O2 consumption and toxicity, oxidative stress and fatty acid oxygen measurement.


Cellular metabolism involves a series of interconnected biochemical reactions catalysed by enzymes inside a cell, better known as metabolic pathways. These reactions convert a substrate molecule or molecules through a series of metabolic intermediates, eventually yielding a final product. These processes allow organisms to grow and reproduce, maintain their structures, and respond to environmental changes.


Amsbio has assembled an unmatched collection of high quality, ready-to-use proteins and cell lysates for use in cell metabolism research. This includes a comprehensive range of compartmental total proteins specifically isolated from cell membrane, cytoplasma, cytoskeleton, nucleus and mitochondria. These focused cell lysates are available from human and many mammalian species / tissue types.


In addition, Amsbio offers cost effective, ready-to-use assays and ELISA kits offering high quality and quick detection of most of target proteins. Available for use with a wide range of sample types and species, these cutting edge kits use unique technologies to study various research areas including apoptosis, DNA damage, oxidative stress, angiogenesis, enzyme activity and cell migration.


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