This page contains a Flash digital edition of a book.

Te team hopes shortly to publish a research paper outlining this discovery.

Other novel technologies New England Biolabs (NEB) has released the Gibson Assembly Cloning Kit and online primer design tool NEBuilder for the cloning of DNA fragments using the Gibson Assembly approach. Tis kit includes NEB’s robust Gibson Assembly Master Mix and NEB 5-alpha competent E. coli, enabling fragment assembly and cloning in less than two hours.

Gibson Assembly enables the rapid assembly of multiple DNA fragments using a one-step, isothermal reaction. It has become a mainstay in the laboratories of many synthetic biologists and is now catching on in the wider life science community due to its ease-of-use, robustness and flexibility.

“As a leader in the discovery and development of cloning tools and technologies, NEB has embraced Gibson Assembly and further developed its protocol for cloning,” states Dr Bill Jack, director of research at NEB. “While traditional cloning methods remain important standard practices in the laboratory, this alternative method delivers additional speed, flexibility and ease-of-use.”

Te Gibson Assembly Cloning

Kit includes protocols for primer design, and has been optimised for the assembly of one or more DNA fragments into a linearised vector.

NEB is an industry leader in the discovery and production of enzymes for molecular biology applications and now offers a large selection of recombinant and native enzymes for genomic research. Te company is expanding its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, cellular analysis, epigenetics and RNA analysis.

Meanwhile Merck Millipore’s

ubiquitous chromatin opening element (UCOE) expression technology gives major improvements in gene expression in stably-transfected mammalian cells through effects on the structure of chromatin. UCOE expression technology prevents transgene silencing and gives consistent, stable and high-level gene expression irrespective of the chromosomal integration site.

Te expression elements are small DNA elements - isolated from the area around house-keeping genes, which need to be active most of the time - that create a transcriptionally active, open chromatin environment

Roche and Agilent sort future of NimbleGen R

oche and Agilent Technologies have signed an exclusive agreement to provide continued service to NimbleGen microarray customers as Roche

phases-out its NimbleGen array production and services. Researchers using NimbleGen microarrays for

all applications, including comparative genomic hybridisation, chromatin immunoprecipitation-on-chip, DNA methylation, and gene expression can transition to Agilent arrays, effective immediately, with minimal disruption. The similarities of the technologies and products

from both companies provide an optimal transition path and the ability to run Agilent microarrays on the

NimbleGen MS 200 Microarray Scanner. “This global collaboration provides our customers

with a confident and straightforward solution to move from NimbleGen to Agilent microarrays,” said Dan Zabrowski, head of Roche Applied Science. “With Agilent as a leading global supplier of microarray technology, we are convinced researchers will be provided with the highest compatibility to NimbleGen products and services, and believe that they will continue to receive the exceptional service and support they have come to expect.” “We are working closely with Roche to help

customers make the transition to Agilent microarray

products,” said Robert Schueren, vice president and general manager of Agilent’s Genomics Systems division. “Our field service personnel are working directly

with individual researchers to help convert their NimbleGen designs, and they will continue to provide enhanced service and support throughout the transition period and beyond. Additionally, Agilent is also enabling customers to read their arrays on NimbleGen scanners, eliminating the need to invest in capital equipment.”

More information about the transition is available at

Fig. 2. An example on the user interface when correlated genes are searched for.

around an integrated transgene, maximising its potential to be transcribed into protein, irrespective of the position of the transgene in the chromosome.

Merck Millipore cites a number of advantages with this technology: over 50 per cent of UCOE-derived clones have higher expression than the best non-UCOE-derived clones; UCOE-derived vectors produce substantially more expressing clones than non-UCOE-derived vectors; cell lines are stable over 130 generations; high-yielding cell

Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52