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chromatography • spectroscopy 21

Chromatography solutions accelerate research efforts

Suppliers are launching new chromatography solutions that increase the speed, accuracy and sensitivity of research efforts. Sean Ottewell reports.

Les fournisseurs lancent actuellement de nouvelles solutions de chromatographie qui font augmenter la vitesse, la précision et la sensibilité des efforts de recherche. Un article de Sean Ottewell.

Lieferanten führen neue Chromatographielösungen ein, die die Geschwindigkeit, Genauigkeit und Empfindlichkeit der Forschungsbemühungen erhöhen. Sean Ottewell berichtet.


hermo Fisher Scientific used Pittcon in March to showcase its Dionex

UltiMate 3000 biocompatible rapid separation (BioRS) liquid chromatography system. Tis combines the performance advantages of ultrahigh-performance liquid chromatography (UHPLC) with the corrosion resistant flow path demanded by the analysis of proteins, peptides, nucleic acids and biotherapeutics.

“We’ve designed this system to accelerate research into promising biotherapies, such as monoclonal antibody variants, and also to support biopharmaceutical research and QA/QC, including glycan analysis,” said Wulff Niedner, director, HPLC product marketing for Termo Fisher Scientific. “Tere is strong demand for a true high- performance biological separation platform, offering capabilities beyond reverse phase UHPLC. Tis combination of state-of-the-art technology and low-dispersion flow path is engineered to provide the high-peak capacity and sensitivity needed to analyse complex biomolecules.”

Te system was developed for reliability and durability, even with harsh solvents used for many bioseparations. Te flow path is built of corrosion-resistant materials, enhancing robustness and extending the life of biocolumns, even under high salt or extreme pH conditions. In addition, the materials used are designed to significantly reduce the risk of analytes interacting with internal surfaces of the flow path, protecting data quality. Te UltiMate 3000 BioRS system is powered by UltiMate 3000 rapid

Fig. 1. The new advanced polymer chromatography (APC) system is up to 20 times faster than traditional gel permeation chromatography (GPC).

separation technology to support the high pressures required to separate many bioanalytes on high resolution UHPLC columns. Te system is engineered to operate at pressures as high as 1000 bar (15,000 psi) and flow rates up to 8 ml/min. Tis wide flow/pressure footprint supports many biomolecule characterisations, including development, identification and quality control. Te tool-free biocompatible Termo Scientific Dionex Viper fingertight fitting system is designed to facilitate connections without dead volume to combat peak-broadening effects.

Te modular system offers many configuration options to accommodate most large molecule workflows. Tese include binary, quaternary and dual gradient mobile phase delivery, as well as parallel and tandem LC configurations.

Termo Fisher Scientific also chose Pittcon 2013 to introduce columns developed specifically to complement the performance of the UltiMate 3000 BioRS system: the MAbPac SCX-10 RS column is designed for UHPLC separations of monoclonal antibodies, while the NAPac PA200 RS column can be used to analyse single nucleic acids and isomers to yield important structural information.

Both feature PEEK linings, providing highly inert flow paths designed to remain stable at UHPLC pressures, and ultra-stable packed beds designed for excellent retention reproducibility and long life.

Another biocolumn choice is the new Termo Scientific Dionex GlycanPac AXH-1 column, designed to enhance glycan analysis

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