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Immunomagnetic separation

Lab M has extended its immunomagnetic separation range with the launch of Captivate O121. This is for the detection and isolation of Escherichia coli O121, a non-O157 Shiga Toxin-Producing Escherichia coli (STEC) that is associated with foodborne infection and is one of the ‘big six’ organisms in meat products for which testing is required in the USA. Although the most common serogroups of STEC in

Europe and the USA are E. coli O157 or O26, the incidence of other serotypes is increasing. The six major non-O157 STECs identified in the USA are O26, O45, O103, O111, O121 and O145. One of the challenges with E coli O121 in particular

is that it is not biochemically unique. Therefore it is difficult to isolate from other STEC strains using conventional culture methods, whereas the use of IMS supports the specific isolation of E coli O121. Immunomagnetic separation is now written into

US Department of Agriculture (USDA) methodology for the detection and isolation of non-O157 Shiga Toxin- Producing (STEC) E coli in meat products and so there is renewed emphasis in the use of this technique. Immunomagnetic separation is the only process

that can be used for the specific isolation of E coli O121 in which a viable microbiological culture is obtained. Antibodies specific to the O121 serogroup have been attached to the super paramagnetic particles via a covalent coupling process. Captivate O121 was developed and tested using faecal samples and pathogenic outbreak strains. Lab M’s expertise in immunomagnetic separation

dates back many years and Captivate is one of the company’s best selling product ranges around the world. As well as producing specific sets of antibody-coated paramagnetic particles for separating and concentrating targeted microorganisms, the company also offers a highly respected consultancy and custom coating service.

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Orbital shaker helps accelerate protein precipitation sample preparation

Porvair Sciences’ MicroShake orbital shaker can be used to accelerate sample preparation when using the company’s popular p3 protein precipitation plates. By simply placing a p3 plate on the MicroShake

dedicated plate holder and agitating for less than 30 seconds - rapid and complete mixing of serum samples with acetonitrile is ensured. The MicroShake has been rigorously tested with p3 plates to ensure that no breakthrough of supernatant liquid can

occur and has been shown to be the fastest way to ensure protein precipitation throughout the sample. The MicroShake uses a 15 volt power supply

and is fitted with a speed adjuster. This compact, reliable and quiet unit is similar in size to a standard format microplate and will fit on any bench, in any fume hood or even the deck of your liquid handler. For added peace of mind and to eliminate any risk of contamination, the p3 plates can be fitted with a re-usable silicone cap mat. A simple adjustment enables the MicroShake to be used with any standard footprint microplate. The p3 plate is proven to eliminate the mess and complex sample preparation (no centrifuging, no vortexing) associated with traditional protein precipitation sample preparation techniques. Based on the industry standard Microlute design, the p3 plate enables 96 samples to be prepared simultaneously.

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Balances provide precision weighing

The PGL series of balances from Adam Equipment withstands the rigours of a laboratory or field setting while delivering precise results quickly and easily. With all the valuable features of a precision balance and convenient portability, PGL balances come in three pan sizes suited for small to large weighing tasks. The PGL family consists of 12

models. Small pan PGL balances offer (145 x 125mm) capacities of 200g-300g and a readability of 0.001g. The medium-pan models (192 x 192mm) have capacities of 2000g-15kg and readabilities of 0.01g -0.1g. With capacities of 10kg- 30kg and a readability of 0.1kg, large-pan PGLs (400 x 300mm) are ideal for weighing bigger samples. All PGLs feature Adam’s ShockProtect overload

Fig. 1. The Captivate O121 immunomagnetic separation from Lab M.

protection to help prevent damage to internal components that could occur from placing excessive weight on the pan. The balances are equipped with up to 12 weighing units and have application modes for parts counting, per centage weighing, specific

gravity/density determination and checkweighing. An all-metal casing and stainless steel pan make the PGL simple to clean. The easy-to-read backlit LCD displays date and time, while an RS-232 interface facilitates data communication. Power is provided by a standard AC adapter and rechargeable battery pack.

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Adam Equipment is based in Danbury, CT, USA.

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