TESTING 31
Figure 3: C-Cube camera.
legs or arms the week before the beginning of the study. Moreover, no waxing/shaving the day before the appointment was allowed. Volunteers applied a cream containing a Salinicoccus hispanicus lysate filtrate versus a placebo in contra-lateral. Both creams were applied in twice-daily massage for four weeks.
Dermatoscopic camera For KP acquisitions, a dermatoscopic camera C-Cube (Pixience, France) was used (Fig 3). This calibrated color camera allows reproducible measurements and produces 16 x12mm images with a 10 mega pixel resolution. The camera was used with its non-glass cap avoiding any skin contact in order to eliminate any artifacts during image capture and checked that shape and volume of the KP remained normal and were not flattened. Moreover, using a technique called photometric stereo, we analyzed multiple images under different lighting conditions to produce 3D images. Combined with a geometric calibration, elevation(z) information at every pixel was obtained. Both 2D mode
Figure 4: Silflo application.
Figure 5: Dermatop.
Figure 6: C-Cube image.
and 3D modes were used in this study. Image J software (National Institute of Health) was also used in order to extract erythema (color threshold) or dryness (gray level threshold).
Silflo and fringe projection Replicas were done using Silflo (Monaderm, Monaco) (Fig 4). A fringe projection system Dermatop (Eotech, France), equipped with its HE-50 lens, was used to realize the 3D acquisition of replicas (Fig 5) with the following
Figure 7: Zoom.
parameters: field of view 40x30x20mm, a 35µm lateral resolution and a 4µm vertical resolution.
Software In order to compare and find the best solution, two software packages were assessed for quantification.
Aeva Software provided with Dermatop (Eotech, France) A 20 x 20 mm area was extracted from the 3D acquisition and a polynomial filter of
Table 1: KP topographic parameters Sa
Sq 65µm Figure 8: 2D image. March 2020 81µm Sz 348µm Figure 9: 3D image. PERSONAL CARE NORTH AMERICA
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