Chromatography 9 Inlet solvent filters maximise efficiency and minimise waste
In the world of analytical chemistry, precision and efficiency are paramount. Microsolv’s Last Drop Mobile Phase Inlet Solvent Filters are for laboratories seeking to optimise solvent usage and protect valuable instrumentation.
These filters are designed to draw nearly every drop of solvent from the bottom of your reservoir, significantly reducing waste and ensuring consistent mobile phase delivery. Unlike traditional filters that leave behind unusable solvent.
What sets these filters apart is their dual material options: passivated stainless steel for robust filtration and PTFE for chemically inert applications. The PTFE version even provides a visual cue for replacement, turning cloudy when clogged—an elegant solution for proactive maintenance.
Whether you’re running HPLC, UHPLC, or LCMS systems, Microsolv’s Last Drop filters help protect your columns and instruments from particulate contamination while maximising solvent efficiency. This not only saves money but also supports greener lab practices by reducing chemical waste. Upgrade your lab’s performance today—because every drop counts.
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Versatile GPC/SEC viscometer
Enhanced analysis of tryptophan and its metabolites in the kynurenine pathway
Tryptophan functions as a building block in the biosynthesis of proteins and is a central precursor of the kynurenine pathway which leads to the production of nicotinamide adenine dinucleotide (NAD) due to the intermediate quinolinic acid. Due to its importance accurate analytical monitoring of tryptophan and its metabolites is desirable.
The coordinating compounds in the pathway pose a challenge for conventional analytical setups, as nonspecific absorption leads to peak tailing and reduced recovery. A new Application Note outlines a robust method for the quantification of all relevant metabolites in the kynurenine pathway. The approach uses a bioinert YMC Accura Triart C18 column, designed to minimise undesired interactions and ensure reproducible performance across complex biological matrices.
Method optimisation using different column hardware types
Testa Analytical’s high-performance viscometer is engineered to integrate seamlessly with any commercially available GPC/SEC system, offering researchers a flexible, high-sensitivity solution for polymer and protein analysis.
Viscometry remains a key technique for gaining structural insight into macromolecules in gel permeation chromatography (GPC) and size exclusion chromatography (SEC). Unlike conventional detectors, Testa Analytical’s viscometer has been specifically designed to overcome the limitations often encountered with traditional systems.
“While the GPC/SEC market once had several viscometer options, today ours is the only one that can be design-optimised to work with any existing system,” said Carlo Dessy, Managing Director of Testa Analytical.
Thanks to its unique architecture, the detector achieves ultra-low signal noise, resulting in a more than tenfold increase in sensitivity compared to other commercial viscometers. This makes it ideal for low-concentration or low-molecular-weight samples - including polymers, proteins, and polysaccharides - and enables reliable performance even at lower flow rates or in semi-micro GPC/SEC setups.
Another advantage lies in its baseline stability. The viscometer returns to baseline automatically without the breakthrough effect that often affects other models, regardless of how many columns are used in the system - eliminating the need for further adaptation.
For labs seeking robust performance and broad system compatibility, Testa Analytical’s viscometer offers a proven, flexible solution to enhance the accuracy and efficiency of macromolecular analysis.
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Figure 1 illustrates the comparison between conventional stainless-steel and bioinert coated YMC Accura hardware. While xanthurenic acid (XA) and kynurenic acid (KA) are hardly affected by the stainless-steel hardware, quinolinic acid (QA) and picolinic acid (PA) show massive peak tailing and reduced recovery. By using the bioinert coated YMC Accura Triart C18 column, peak tailing can be significantly improved and a higher peak sensitivity can be achieved.
Figure 1: Analysis of tryptophan and its metabolites using stainless-steel column hardware (top) and a bioinert coated YMC Accura Triart C18 column (bottom).
Quantification of tryptophan and its metabolites in teleost brain tissue using LC- MS/MS
For the application of the method to a biological matrix, teleost brain tissue was extracted with 1 mL methanol. Centrifugation removed protein components from the
sample.The resulting supernatant was evaporated and reconstituted in a 50:50 mixture of eluent A and B. All target compounds are reliably quantified in the biological sample (see link below).
Conclusion
The bioinert coated YMC Accura Triart C18 column enhances sensitivity and delivers sharper peaks for the analysis of tryptophan and its metabolites. This Application Note confirms the method’s suitability for complex biological matrices and demonstrates its robustness under real-world conditions.
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