26 Accelerating Assay Development with Lyophilisation-Optimised qPCR Reagents
Applied Biosystems has released the TaqMan™ 2.5X Lyo-Ready 1-step qPCR Master Mix with excipient, a ready-to-use formulation that can be easily incorporated into lyophilisation processes. This reagent undergoes functional testing for lot-to-lot reproducibility and is manufactured in an ISO 13485-certifi ed facility to maintain high quality standards. The product also includes lyophilisation guidelines to facilitate a fast and reliable transition from liquid to lyophilised form.
Newcomers to lyophilisation may face diffi culties in fi nding a suitable excipient and protocol for their qPCR-based assays. However, the TaqMan™ Lyo-Ready Master Mixes offer optimised performance both before and after lyophilisation, and come with guidelines to support molecular diagnostic kit manufacturers. This can help to shorten the path to commercialisation.
“The lyophilisation phase can be a challenging part of any workfl ow to navigate, even for those with experience,” said Kathy Latham, Senior Director, qPCR Reagents at Thermo Fisher Scientifi c. “Our Lyo-Ready Master Mixes take the guess work out of choosing an excipient and provide consistent performance. Taken together, we believe this will help our customers shorten the path from an idea to a commercial lyophilised product.”
The Lyo-Ready Master Mixes offer a fast and simplifi ed workfl ow, reducing the time and cost required to develop robust lyophilisation protocols by 50%. These mixes are optimised to ensure consistent and reliable performance both before and after lyophilisation, offering precision performance. They are also tolerant to challenging conditions and are compatible with purifi ed samples and crude lysates commonly found in biopharmaceutical, molecular diagnostic, and research applications.
More information online:
ilmt.co/PL/Gbnj 59871pr@reply-direct.com
IR Hotplate/Stirrer Delivers Superior Heating and Mixing
The latest infrared heating technology has been used in the design of the WH280-NH hotplate-stirrer from Wiggens. Thanks to this radiation heating technology, the Wiggens WH280-NH can be used for rapid heating up of various shaped vessels, including round-bottom fl asks, and the solution inside can be heated up to a maximum of 450°C in a very short time.
The new Wiggens hotplate stirrer enjoys the benefi ts of a Schott glass ceramic surface with exceptional chemical resistance, a high surface quality, and a resistance to temperature shocks of more than 700°C, providing users with an optimal solution compared to conventional heating surface materials. The poreless smooth surface enables even the most stubborn dirt to be easily cleaned. The high infrared permeability ensures that the heating energy is transferred quickly and with very limited loss, therefore, it heats liquids fast and saves time and energy. Intelligent Cascade Control enables self-optimising to reach perfect temperature stability. If the Pt-100 sensor is connected, temperature-controlled work with stability of +/- 2°C can be reached in most applications.
In addition to rapid heating capability and superior temperature control, the WH280-NH also offers well controlled gentle to intense mixing of liquids, and is also suitable for large volume viscous media.
The stirring and heating can be controlled separately by convenient turning knobs. All functions can be viewed and monitored on a bright touch screen, including most frequently used parameters, such as, working temperature, stirring speed, working time, and safety temperature. Memory function for stirring speed and temperature setting makes it convenient to start the experiments with fi xed conditions. A residual-heat display protects the users from burning their hands.
With the aforementioned benefi ts, and many other features, the Wiggens hotplate stirrer is the optimal solution for heating and mixing of liquids in labs, for gentle to intense applications.
More information online:
ilmt.co/PL/gP3P and
ilmt.co/PL/Q2Qe 59072pr@reply-direct.com
Dual Capability Coded Tube Reader
Ziath announce the launch of a new dual capability coded tube reader - the Duo. This affordably priced addition to the Ziath range combines the proven 2D-barcode reading technology of the Uno single tube reader with the fast accurate linear barcode reading of the company’s Cyclops adaptor. The new Duo tube reader provides a compact benchtop solution for fast and accurate reading of both code types.
The Duo tube reader acts like a ‘keyboard wedge’, inserting the numeric barcode value from the tube it has scanned into any open program on your PC desktop. This high-quality tube reader captures and decodes 2D barcodes from the base of tubes in just one second and can read most commercially available tubes with Datamatrix codes on their bases. The additional linear barcode scanner is designed to read linear rack barcodes from the side of whole racks of tubes or the linear code, if present, from the side of an individual tube.
Designed as a single, self-contained device - the Duo dual capability coded tube reader requires just one USB led to connect and power it from your PC.
Durably constructed, the Ziath Duo is a truly portable reader which can be held and operated in one hand; leaving the other free for tube handling.
More information online:
ilmt.co/PL/AwLO 58446pr@reply-direct.com
Maximising Effi ciency at -80°C: Join the 2023 International Freezer Challenge
Global warming is a pressing issue for humanity, recently highlighted by events in Sharm el-Sheikh. While addressing this challenge requires collective action at the UN level, individuals can also make a difference. Conversations about sustainability often focus on energy consumption, and laboratory equipment is no exception. Despite being energy effi cient, ultra-low temperature freezers still consume a considerable amount of energy to maintain a constant temperature of -80°C 24/7 for years.
The Freezer Challenge 2023, aimed at recognising the most sustainable cold storage solutions, is back and brought to you by the non- profi t organisation My Green Lab and the International Institute for Sustainable Laboratories (I2SL).
Join the Freezer Challenge 2023 and earn points by implementing good management practices, fi ne-tuning temperatures, and sharing information about effi cient practices. The challenge rewards those who make the greatest contribution to energy conservation and improved sample storage. By optimising your own refrigeration equipment, you’ll be part of a larger effort to raise awareness about energy use in the laboratory.
The Freezer Challenge has made a signifi cant impact since its launch in 2017, leading to a global savings of over 24 million KWh of electricity through improved cold storage practices.
Eppendorf is committed to environmental sustainability and has shown this commitment by supporting the International Freezer Challenge every year since 2018, marking the 6th consecutive year of support.”
Register free of charge. More information online:
ilmt.co/PL/k1d8 59712pr@reply-direct.com
INTERNATIONAL LABMATE - APRIL 2023
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