Chromatography 17 Oligonucleotide Separations with RP Phases
Therapeutic oligos represent an important area of research in the pharmaceutical industry today. These drug candidates are typically 8-50 nucleotides long and contain single-stranded DNA or RNA. Oligonucleotides are typically analysed using ion-pairing chromatography. Oligonucleotides are easily degraded via phosphodiester-cleaving enzymes. However, with a thiophosphate modified phosphate group, the stability significantly increases. Phosphorothioate modified oligos will have a stereocenter at each modified phosphate group, leading to 2n-1 diastereomers, all with an individual retention time in the chromatography. For a 20-nucleotide long oligo, that’s 524 288 species present. The partial resolution of these species results in peak broadening, further complicating chromatographic separation. To resolve the problem, ion-pairing agents with longer alkyl chains can be used.
In a study of the influence of stationary phase and ion-pairing agent on the separation of oligos, Kromasil Phenyl showed to be a very good choice when separating oligonucleotides in general, exhibiting very sharp peaks, and good selectivity.
More information online:
ilmt.co/PL/XvKy 48275pr@reply-direct.com
Innovative Column Solutions for BioLC
With over 10 years experience in the production of BioLC solutions, YMC announces new and innovative products for peptides, proteins and especially antibodies.
For RP separations YMC-Triart Bio C4 is a widepore phase for (U) HPLC. It offers the perfect solution of outstanding selectivity for peptide/protein analyses as well as antibody separations with its 300 Å pore size. Method flexibility is enhanced due to high pH (1-10) and temperature stability (up to 90°C). The superior reproducibility guarantees reliable BioLC QC.
Extraordinary selectivity for antibody analysis is provided by YMC-SEC MAB. Simultaneous analysis of the antibody together with its fragments and aggregates in just one run is possible. YMC-SEC MAB is the ideal choice for users developing QC methods due to its outstanding reproducibility.
Effective separation of antibodies, ADCs and proteins is provided by the hydrophobic interaction column BioPro HIC BF. The butyl bonded polymer phase provides cost-efficient long-term stability. The excellent reproducibility assures a consistent QC. Analysis throughput is improved with virtually no carryover effects.
More information online:
ilmt.co/PL/Kx4g 48177pr@reply-direct.com
Automation of Analytical Sample Preparation
Eprep’s new desktop instrument, which allows robotic comprehensive sample preparation for the analytical (chromatography) laboratory with effortless accuracy and consistent results every time.
Described as the ‘go-to’ instrument for analytical laboratory sample preparation, Andrew Minett, General Manager at Eprep said: “The Eprep suite of innovative technologies brings comprehensive and affordable robotic sample preparation to the analytical laboratory. Sample preparation techniques have not kept up with the efficiency of modern analytical equipment. The ePrep workstation is a game changer, providing a simple cost-effective solution for automation of laboratory sample preparation.”
Simple to program and operate, ePrep allows even complex Workflows to be configured in minutes. The product offering comprehensively addresses sample preparation techniques in an analytical laboratory. ePrep has been designed specifically to bring sample preparation to the analytical (chromatography) laboratory.
More information online:
ilmt.co/PL/Y63p 47205pr@reply-direct.com
New Flash Purification System and Columns Announced
Biotage a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of Biotage®
Selekt, a new flash purification system and the new Biotage® Sfär family of flash purification columns.
Together these two new products provide both organic and peptide chemists the opportunity to improve their separations. The Biotage®
Selekt flash purification system is a very compact instrument with a small bench top footprint. Biotage® ADVANCED Sfär columns
can be mounted either on the side or on the front of the instrument, again saving space in the laboratory. The instrument’s high flow and the high-pressure capability enables purification to be performed at a faster rate. Other features also help to increase the rate of purification whilst reducing solvent usage. Biotage®
Selekt features a large touch screen with a completely redesigned and flexible
interface, ensuring that the intuitive controls are easy to use and reducing the time needed for training. In parallel with the new system comes Biotage®
Sfär, a complete new portfolio of flash purification columns with various media
and sizes ranging from 5 g to 350 g. These columns use spherical silica with a high surface area and consequently a higher loading capacity – enabling chemists to select a smaller column to perform the same separation, reducing both the amount of solvent used and the cost of the separation.
Sfär columns are flexible, chemists can liquid or dry load the columns as they wish, as with the current Biotage columns. The new columns can be opened, and used in conjunction with Biotage Samplet® to Biotage.
Biotage® cartridges for optimum chromatography - unique “The combination of the Biotage® Selekt system and Biotage® Sfär columns is a modern and state of the art total Flash
Chromatography solution,” said Tobias Nordin, Global Product Manager, Flash Purification, Biotage. For further information visit
www.biotage.com.
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