Laboratory Products 41
Report on Nanoparticle Tracking Analysis use in the Brno Veterinary Research Institute
New Smart Infrared Loop Steriliser
For highspeed annealing and sterilisation without gas fl ame, the SteriMax Smart from WLD-TEC is ideal for all laboratories, anaerobic environments and safety cabinets. This innovative Steriliser uses specifi cally focused infrared light which generates an IR hotspot for sterilising inoculating loops at a temperature of 650° to 1100°C in only 5 to 10 seconds immediately and without any preheating.
Features Include: instantly ready to work without any warm-up; auto-start through touchless IR-Sensor technology; adjustable sterilisation and cool-down timers for two users by front panel operation; annealing tube made of special quartz glass or optional wear-resistant ceramics; very simple cleaning of the device and annealing tube; effi cient use of energy and minimal heat dissipation thanks to Thermo Control; cool -touch housing made of stainless steel and safety glass.
For additional information and specifi cations on the SteriMax Smart go to the WLD-TEC website.
27424pr@reply-direct.com ADVERTORIAL
The central theme of Dr Jaroslav Turanek’s research group (Department of Pharmacology and Immunotherapy), at the Veterinary Research Institute in Brno, Czech Republic, is to apply synthetic and bioorganic chemistry. This work is performed in collaboration with King’s College London and the Institute of Organic Chemistry and Biochemistry, Prague, for the design and construction of therapeutic nanoparticles to develop drug delivery systems and nanocarriers for construction of recombinant vaccines.
In parallel, the research group of Dr Miroslav Machala (Department of Chemistry and Toxicology) at Veterinary Research Institute focuses upon environmental nanoparticulate pollutants. Characterisation of airborne particles is conducted using electron microscopy, but in vitro tests on cell culture require knowledge of the real structure of nanoparticles in the tissue culture medium.
Detailed particle distribution and kinetics of aggregation in this heterogeneous system is impossible to obtain using electron microscopy and hence Nanoparticle Tracking Analysis, NTA, is the method of choice. It is noted that some metastable aggregates can disaggregate due to high dilution of the sample required for NTA analysis. For this reason, Dynamic Light Scattering, DLS, and NTA are used as suitable complementary methods in the laboratory.
The Malvern NanoSight NS500 system is used in the VRI laboratories as alongside other techniques including DLS (Zetasizer Nano ZS), static light scattering, Gel Permeation Chromatography, electron microscopy and Field Flow Fractionation.
Explaining their choice of NanoSight, Dr Turanek said: “We chose NTA as a convenient and rapid method for characterisation of nanoparticles in heterogeneous preparations like liposomes and their complexes with proteins, DNA and polysaccharides. A set of these techniques is used for the complex characterisation of the structure of the nanoparticles, the kinetics of their preparation, the dynamics of morphological transformation and, fi nally, their stability. NTA perfectly fi ts our needs and has become a standard method in our methodological portfolio. The most advantageous feature of NTA is that it makes it possible to visualise each nanoparticle and then to obtain more detailed size distributions based on individual particle measurements. DLS is used as precise complementary method for the characterisation of nanoparticles below 20 nm for proteins and other biopolymers. Combination of these two methods, NTA and DLS, with separation methods (GPC, FFF) and electron microscopy is preferred to get the full insight to structure and dynamics of nanoparticles in our sample systems.”
28038pr@reply-direct.com
Free Download of SpyLight®
-Software
SpyLight is a software for visualisation and documentation of process- relevant data. Communication occurs over RS232, RS485 or TCP/IP. The recorded data are displayed over a time axis. The axes of the diagram are freely
scalable in the process. A zoom function simplifi es the graphics evaluation of individual time sections. The software provides menu prompting in German and English.
SpyLight is quick and easy to install. The software can be downloaded free of charge at
www.huber-online.com. To install the software simply double-click the setup fi le and follow the instructions. The installation program is easy to use and requires no special knowledge. The SpyLight requires hardly any hard disk space. The software is compatible with the Pilot ONE controller as well as with the cheaper MPC controller.
More information can be found on the website, or in our catalogue which can be downloaded at
www.huber-online.com or requested free by emailing
info@huber-online.com.
28781pr@reply-direct.com
Free Firmware Update for Huber Temperature Control Units
A new fi rmware is available for Huber temperature control units with ‘Pilot ONE’ controller. The update comprises several new functions, for example a user administration with several menu and authorisation levels. The new function can be used to create password- protected user accounts. The access rights to these are freely confi gurable. In this way, key settings and operating parameters can
be protected against inadvertent changes, and access may be restricted if required. Another new feature is the option to save process data directly to a connected USB data carrier.
Want to stand out from the crowd?
The data is saved in 5 second intervals as a universal CSV fi le which can be easily evaluated and edited, for example with Microsoft Excel®
. The fi rmware is updated by means of
the ‘Pilot ONE Flasher’ software available for download on
www.huber-online.com. The installation package comes with detailed instructions.
Contact:
sales@intlabmate.com
28783pr@reply-direct.com Peter Huber Kältemaschinenbau GmbH
Werner-von-Siemens-Strasse 1 • Phone +49 (0)781 9603-0 •
77656 Offenburg
info@huber-online.com
www.huber-online.com
1654ad@reply-direct.com
• Working temperatures: -40°C to +200°C • Incredibly compact dimensions • Unique thermodynamics • Highest cooling power density [w/l] • Brilliant 5.7“ touchscreen controller • Ethernet, RS232 and USB interfaces • Natural refrigerant propane R290
-125...+425°C
Petite Fleur and Grande Fleur are the smallest temperature control systems in the Unistat range. Thanks to their compact form, the units can be operated in confined spaces, for example laboratory fume hoods. Both models are predestined for the temperature control of small and medium sized research reactors.
IMPRESSIVE ... SMALL AND POWERFUL
WWW.LABMATE-ONLINE.COM
Petite Fleur ® meets Grande Fleur ®
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56
