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Digest 12 samples in <20 minutes


Ready for a game change in sample preparation? Multiwave GO, the latest masterstroke from Anton Paar, is built to simplify and accelerate your everyday laboratory work.


MARCH/APRIL 2014 - VOLUME 20 ISSUE 2 1773ad@reply-direct.com


Multiwave GO is a digestion system for routine applications, featuring a sophisticated 5 kg lightweight rotor with 12 pressure-activated-venting vessels. Based on the system’s DMC Directed Multimode Cavity – the new self-adjusting microwave fi eld invented by Anton Paar – highly effi cient heating is possible in an extremely small-footprint system. For effi cient reaction control the internal vessel temperature of each vessel is controlled via an IR sensor. Due to the system’s TURBO cooling, unique cooling times as short as 8 minutes are possible for a fully loaded twelve- position rotor, ensuring superior sample throughput. Multiwave GO is extremely intuitive to operate, robust and cost-saving.


Ready, steady … GO! Quickly and easily access your fi rst successful sample preparation with Multiwave GO.


OFC_LA_APR_14.indd 1 25/03/2014 15:35 Product website: www.anton-paar.com/multiwave-go 1773ad@reply-direct.com


In this Issue Also available at:


labmate-online.com/ejournal/ Chromatography


Paul Ng, Kiran Kaur, Jonathan Kohn, Anna Quinlan, and Jeff Habel


4-13


Protein Purifi cation Workfl ow Development Using Bio-Rad’s NGC™ Chromatography System


4


Monolithic Silica Columns for Simple and Fast LC-MS Analysis of Antibiotics in Mammalian Tissue and Body


Fluid and in Pharmaceutical Formulations Stephan Altmaier


10


Mass Spectrometry & Spectroscopy Oliver Büttel


14-19


Background Correction In AAS – Can You Really Trust It As Much As You Do?


14 Head Office - International Labmate Ltd


Oak Court, Sandridge Park, St AIbans, Hertfordshire AL3 6PH, United Kingdom. Tel: +44 (0)1727 855574 • Fax: +44 (0)1727 841694 Amend your subscription: subscribe@intlabmate.com Other enquiries: info@intlabmate.com • sales@intlabmate.com ISSN 0143-5140


Publishers of: International Labmate, Labmate UK & Ireland, Lab Asia, Chromatography Today, International Environmental Technology, Asian Environmental Technology, Pollution Solutions, Petro Industry News and Measurement Analysis China


Laboratory Products News Asia


Gwyneth Astles 2


Business Opportunities Europe & Middle East Heather Hobbs


Publisher Michael H. Pattison


Director of Publications Marcus Pattison


Director, Asia Pacific Marie Izzard


michael@intlabmate.com marcus@intlabmate.com marie@intlabmate.com.au


North American Area Manager Carol Hardy


Sales & Marketing Manager Chris Jarvis


European Sales Manager John Hobbs-Morris


Area Manager Carly Hider


Online Manager Rob Parker


Guide Manager Sam Jackson


Guide Assistant Joanna Hemmings


Reader Services Manager Sue Petts


carol@intlabmate.com chris@intlabmate.com john@intlabmate.com carly@intlabmate.com rob@intlabmate.com sam@intlabmate.com joanna@intlabmate.com sue@intlabmate.com Editor Gwyneth Astles


Contributing Editor Heather Hobbs


gwyneth@intlabmate.com heather@intlabmate.com


Contributing Editor - Separation Science Trevor Hopkins


Production Manager Robert Battell


Production Team Heather Vincent Jill Mitchell Caroline Belt


Finance Controller Chris Brown


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Maureen Woodhouse


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Market Research trevor@intlabmate.com robert@intlabmate.com


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maureen@labmate.net Marstonbury Ltd


Printed by Magnum / Published by International Labmate Ltd.


Published: January/February, March/April, May/June, July/August, September/October, November/December


Lab Asia is distributed to scientists, technologists, laboratory technicians and environmentalists throughout the Pacific Rim, free of charge.


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North American Office: Carol Hardy - International Labmate Ltd 333 Colony Blvd. #194 The Villages, FL 32162, United States Tel: +(1) 623-869-0184 Fax: +(1) 623-321-9200. E-mail: carol@intlabmate.com


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Tel: (03) 3208 7821 Fax: (03) 3200 2889


Korea: Pearl Yoon-Joo Jin - The Peak Media Co Ltd Suite B-2010, Intellige 2 B/D, 24 Jeongja-dong, Bundang-gu, Seongnam, Gyunggi-do 463-841, Republic of Korea Tel: +82 (0)70 4639 3008 Fax: +82 (0)31 712 3228 Email: pearl@tpmkr.com


International Labmate is published six times per annum in tabloid format. In addition a most comprehensive “Guide to Laboratory and Environmental Equipment” printed in A4 format. It incorporates companies, products and dealers, mailed in May/June.


Although every effort is made to ensure the accuracy of material published, International Labmate and its offices cannot accept responsibility for claims made by contributors, manufacturers or advertisers.


© All rights reserved by International Labmate Ltd. Reproduction in part or whole without written permission is strictly prohibited. Please look after the environment by recycling this magazine


Centrifuges, Stirrers & Shakers Gwyneth Astles


38


International Labmate is a member of the


following trade associations:


Food & Beverage Analysis Gwyneth Astles


30


Life Sciences Gwyneth Astles


20 Special Features Microscopy & Microtechniques Ann P. Wheeler, Neveen A. Hosny & Martin M. Knight 48-50


Spinning Disk Super-Resolution Microscopy – Bringing Super-Resolution in Focus for the Cell Biologist


48 46 2-47


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