Life Sciences Multimode Detection System for Cell and Biochemical Assays Introduced
Promega Corporation have announced the launch of GloMax® Discover, an integrated multimode detection plate reader system. Together with the company’s extensive cell and biochemical assay portfolio, GloMax Discover offers scientists an integrated system to easily study cell signalling and metabolism, while monitoring cell viability, apoptosis, and cytotoxicity, or to study gene expression with a wide variety of reporter assays. The system offers a broad dynamic range for both strong and weak bioluminescence experimental samples, superior luminescence sensitivity and low well-to-well cross talk, enabling users to generate more usable data from each experiment. GloMax Discover provides flexible use of filters for fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance measurements, and seamless integration with industry-leading bioluminescent assays from Promega. The ready-to-use format offers cost and time savings, as less user intervention and reagent are needed for optimisation.
Michael Bjerke, Promega Global Product Manager, Detection, commented: “Scientific discoveries not only require the right type of assay to achieve the necessary sensitivity, dynamic range, and reproducibility, they require the right type of instrument. Instruments with enhanced optical capability, such as our GloMax systems with high quality luminescence, enable superior assay performance and experimental design.”
The GloMax Discover software was developed by Promega with the goal of designing elegant, easy-to-use, and high performance systems in mind. The software provides quick and easy navigation through the control options, preloaded assay protocols, and drag-and-drop protocol customisation. Exporting results is simple, with a variety of options including exporting to a local data network, external flash drive, internet cloud-based server, and Laboratory Information Management System (LIMS). The GloMax Discover software also provides the technical elements of a 21 CFR Part-11-compliant system, to be used with appropriate laboratory workflows.
28881pr@reply-direct.com Range of Low-Retention Tips Developed Specifically for Life Science Researchers
SSI has launched a new comprehensive family of pipette tips designed to provide researchers with the highest level of liquid handling performance.
The Vertex™ Pipette Tips were developed following extensive research to provide users with a high degree of fluid confidence and control while pipetting. SSI internal studies have indicated their tips provide less fluid retention and less sample loss. All Vertex tips are manufactured using SSI’s specially formulated NoStick® resin. Through use of this resin, the inner surface of the tip is considerably more hydrophobic than standard industry tips enabling viscous fluids in particular to be dispensed more completely. The finely tapered tip construction and thinner walls eliminate the need for users to touch off their pipettor. Researchers maintain more pipetting control in general, but especially when working with smaller volumes.
SSI has produced a short video detailing their new Vertex™ Pipette Tips. It can be found on their microsite.
The new range of tips includes bulk, racked, sterile, and EcoPac® (refill insert) options. They are producing Un-Filtered and Filtered varieties in 10µl, 10µlXL, 200µl, 300µl, 1mL and 1250µl sizes.
The Vertex line includes a time-saving EcoPac® Refill System. The new system does not require any tools or special instructions to enable users to move from an empty insert to a fresh set of 96 tips. The Vertex racks were uniquely designed for one-hand operation. A simple press on the front of the lid releases it from the rack; otherwise the lid sits securely closed. The EcoPac refills provide over a 70% savings in total materials (including products and packaging).
27821pr@reply-direct.com Improving Overall Growth and Performance of Primary Neurons
Developed as an alternative to B27 - GS21 from Amsbio is a next generation serum-free neural media supplement, based on the formulation of NS21 Supplement, designed to improve the overall growth and performance of primary neurons.
GS21 has been specifically optimised for the maturation and long-term viability of primary rat and mouse neurons in culture, without the need for co-culture with astrocyte feeder cells. In experimental tests - GS21 has been shown to significantly improve long-term viability of rat and mouse primary neurons in vitro as well as enhance neurite outgrowth of primary neurons in culture. Beneficially GS21 also supports growth and maintenance of primary neurons at low or high cell density plating.
Amsbio is a leading provider of innovative products and services to support all stages of stem cell and neurobiology research from source to storage. Regardless of the application, Amsbio offers products and services to accelerate basic, translational and clinical research.
28889pr@reply-direct.com Automated TruSeq® Stranded Total RNA Library Preparation Method Announced
Eppendorf have announced the release of the epMotion automation method for Illumina’s TruSeq Stranded Total RNA library preparation kit for next-generation sequencing as ‘Illumina Qualified’. ‘Illumina Qualified’ indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually. Sample preparation for next- generation sequencing (NGS) involves extensive manual processing that can affect reproducibility. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labour-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation, brings about a standardised and streamlined process, with significantly reduced hands-on time and minimisation of human errors.
“Eppendorf’s method for TruSeq Stranded Total RNA provides our customers with another great option for high throughput automation,” said Peter Fromen, Senior Director of Product Marketing at Illumina. “Eppendorf is one of the leading providers of automation to NGS users, and we are looking forward to additional methods from them in the near future.”
“The Stranded Total RNA sequencing library preparation using the epMotion minimises our hands-on time while achieving overall high reproducibility. This streamlines and standardises our sequencing workflow, thereby allowing for higher sequencing throughput. We are looking forward to collaborating with Eppendorf on additional automated library prep protocols,” said Dr Wei Chen, Head of the Scientific Genomics Platform at Max Delbrück Centre for Molecular Medicine in Berlin, Germany.
“This method is tailored for 8, 16 or 24 samples of 100 to 1000 ng total RNA and gives sequencing ready libraries in 9 to 12 hours. While this is not much faster than the manual process, the hands-on time is significantly reduced as the automated process needs less than one hour preparation time in total,” said Dr Reza Hashemi, Executive Director of Portfolio Management Liquid Handling at Eppendorf.
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