Life Sciences Genetically Characterised FFPE Tissue Samples
Amsbio has launched CancerSeq™ - a new range of genetically characterised FFPE tissue samples. CancerSeq™ samples are prepared from genomic DNA extracted from the FFPE tumour tissues and validated by Next Generation Sequencing using Illumina’s TruSeq Amplicon - Cancer Panel (TSACP). Available as tissue slides and whole blocks, samples are delivered with the mutation and variation data for 48 cancer-related genes including BRAF, KRAS, EGFR, ALK and TP53.
CancerSeq™ tissue samples provide researchers with the perfect medium for applications including validation of cancer marker mutation related drug candidates; companion diagnostic assay development; cellular localisation of tissue specific mRNA and protein expression; Comparison of the locations of novel genes in different tissues as well as in situ hybridisation, immunohistochemistry, and in situ PCR experiments.
Available from several tumour tissue types, donor clinical history data is provided with each CancerSeq™ sample. In addition, Amsbio offers custom services to characterise your specimens or selected samples from its biorepository of cancer and normal FFPE blocks.
28887pr@reply-direct.com PointMan™ Enriches Low-Level DNA Mutations from Whole Blood
EKF Diagnostics announces a major breakthrough for its PointMan™ DNA enrichment technology for potential use in future cancer testing and treatment.
The first successful results of a collaboration between EKF Molecular Diagnostics and the Institute of Life Sciences at Swansea University have demonstrated the detection of gene mutations in blood from samples archived in the Wales Cancer Bank. The Company’s PointMan™ technology was used to analyse the whole blood of cancer patients diagnosed with metastatic melanoma (skin cancer that has spread) enabling the identification of gene mutations associated with response to drug treatment.
Crucially, the results observed for mutations in the gene BRAF were consistent with the formalin fixed paraffin embedded (FFPE) tissue samples. FFPE being the laboratory standard method to prepare all biopsy samples for pathology review in order to diagnose the cancer. These results have been confirmed by DNA sequencing which had failed to identify the mutations prior to sample enrichment through EKF’s PointMan™ technology.
EKF Molecular’s portfolio of PointMan DNA enrichment products include; BRAF, KRAS, EGFR, NRAS and JAK2. PointMan, is a real-time PCR technology that provides reliable and extremely sensitive detection for cancer mutations. It is highly efficient in amplifying the target sequence of interest, whilst suppressing amplification of the wild-type. The resulting sample is effectively enriched for the mutation, thereby having the potential to offer industry leading sensitivity in a wide variety of sample types, including whole blood. This is demonstrated in the ILS Swansea study.
29011pr@reply-direct.com Precision Heated Hybridisation Ovens
UVP, LLC offers a range of hybridisation ovens with microprocessor controls for maintaining precision heating, rotation and thorough mixing of samples. Models include the HB-500 compact desktop oven for use with small bottles or conical tubes. The HM-4000 features two chambers with shake, rock, roll and rotate motions and accommodates a variety of vessels. HL-2000 combines a hybridisation oven and UV crosslinker. The carousel that holds twenty 35 x 150mm or ten 35 x 300mm bottles. The UV crosslinker utilises shortwave 254nm UV for bacterial destruction or crosslinking prior to hybridisation. Hybridisation ovens and UV crosslinkers chambers are available as separate units.
For more information, go to
http://www.uvp.com/
hybridizationoverview.html.
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Launch of New qPCR Assistant Software
Gilson is pleased to announce the launch of the new qPCR Assistant software that reduces the hands-on sample preparation burden for researchers running PCR and qPCR experiments in translational science and other research areas.
With the increasing need for biomarker validation using qPCR and PCR techniques across a variety of biological samples, researchers are seeking new ways to maximise qPCR sample preparation accuracy and reproducibility, eliminate inherent variability and minimise sources of contamination. The qPCR Assistant is a workflow-focused solution, based on the recently introduced PIPETMAX automated ‘lab assistant’ pipetting station. Developed by molecular biologists, it puts molecular biologists in the driver’s seat by combining an intuitive user interface and versatile qPCR-focused design of experiment workflow with a familiar Gilson PIPETMAN-inside accurate automated ‘lab assistant’.
“I created a qPCR experiment from scratch in minutes with PIPETMAX qPCR Assistant, without having to know any details about liquid handling. The PIPETMAX qPCR Assistant also instructed me on the labware positions, how and what to prepare for each run, and exported a template file for my thermocycler, which made the whole process of creating and running the protocol very time efficient. The optimised qPCR plate setup enabled method and pipetting efficiency and the method traceability provides the reassurance of reproducible results,” said David Dobnik, PhD, GMO detection lab, Department of Systems Biology and Biotechnology, National Institute of Biology, Ljubljana, Slovenia.
Protein Precipitation Sample Preparation Kit
The Combipack™ from Porvair Sciences has been introduced for laboratories tasked with protein precipitation sample preparation. Comprising five colour coded Porvair P3 protein precipitation plates and five 1ml deep well collection plates the Combipack™ contains all the elements required to accelerate the traditionally troublesome procedure at a budget price.
Biological samples commonly contain proteins that interfere with downstream applications. The P3 plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but without the chromatographic sorbent. Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix means that there is no ‘wetting out’ and leakage of the sample through the plate before the application of vacuum. P3 is now commonly used as the protein precipitation plate of choice in many major pharmaceutical companies. Optional drain cap and top cap mats are available for those wishing to use vortex sample mixing.
Manufactured to the standard ANSI/SBS 96-well format footprint all plates in the Combipack™ are fully automation compatible.
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The qPCR Assistant software also generates a traceability output report that provides the experimental design details and plate locations for the thermocycler. Repeating, qPCR experiments, is simplified with a drop-down menu and selecting your previously designed method. In addition, the traceability provides an added level of electronic security helping to eliminate manual errors, maximising reproducibility and further saving the researcher time.
Whether managing 10s or 100s of samples, in an individual lab or across many collaborative sites, the qPCR Assistant software and PIPETMAX solution transforms qPCR experiment efficiency and reproducibility.
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