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aflatoxins on selective binding antibodies they are separated from the matrix and afterwards released by elution with organic solvents. The resulting extracts can be analysed by RP18-HPLC detecting the mycotoxins by fluorescence after post-column derivatisation with bromine or iodine solutions. Often entire ship loads of nuts or similar products cannot be released before the aflatoxin content is determined exactly. The method described here provides representative results in a very short time thus giving the supplier as well as the consumer optimum protection.


CUTTING MILL SM 100 • Feed material: soft, medium-hard, elastic, fibrous • Material feed size: < 60 x 80 mm • Final fineness: 0.25 - 20 mm


• Defined final fineness by use of bottom sieves • 3 different hoppers for different materials • Samples subjected to low thermal stress


MOULDS WHICH GENERATE MYCOTOXINS


ALTERNARIA


The graphic shows a typical chromatogram of a sample containing aflatoxin. It is possible to determine the type of mycotoxin as well as to make an exact statement about the quantitative contamination. the ultra centrifugal mill ZM 200


Amino Acid Analyser Applications for Food and Feedstuffs


The Biochrom 30 is a dedicated amino acid analyser ideal for the busy industrial QC lab running 24/7. The Ninhydrin detection/ion exchange chromatography method is totally compliant with EU commission directive 98/64/EC and AOAC method for the determination of free and total amino acid content in food and feedstuffs.


Both the instrument and the method are robust, easy to use and economical to run. The column has a long life, is acid and base resistant and fully compatible with possible high concentrations of salts. It can be fully regenerated and re-optimised, enabling years of usage, controlling costs and minimising waste. A wide range of sample types can be run and thanks to the online post-column derivatisation of the amino acids with Ninhydrin, the precision on peak area is better than 1.5% RSD.


The peak separation is >95% on common amino acids profile (up to 45 amino acids in a single run with Lithium systems), including Taurine, Ornithine, oxidised amino acids such as Cysteic acid and Methionine Sulfone, and hydroxylated amino acids such as Hydroxyproline and Hydroxylysine. There is a choice of two buffer systems available, both


totally optimised for food and feedstuff analysis including an accelerated buffer system for high sample throughput labs.


Short methods are available for specific applications such as Lysine or Taurine content and there is a range of accessories to analyse Polyamines. Technical and Scientific Support is available worldwide from a dedicated amino acid analysis support team.


Circle no. 403


Supervised From Farm to Fork


According to estimates by the World Health Organisation (WHO), approximately 10% of the population in the industrialised nations become ill each year due to contaminated food. Scrupulous quality controls are not only an increasingly challenging task for health authorities, they also help to prevent scandals in the food industry, and are the best way to regain consumer confidence. Particular emphasis is placed on the identification of pathogens in foods throughout all stages of food processing, from raw materials to consumers, also known by the slogan ‘From farm to fork’.


In order to ensure quality throughout all stages of the production process, Peka Kroef has its own microbiological laboratory, where comprehensive testing is performed around the clock. An initial inspection is performed when the raw materials are delivered. Hygiene checks are performed regularly on all surfaces that come into contact with the products, the air-conditioning systems in production facilities are tested for the presence of airborne bacteria, and wastewater checks are also part of hygiene monitoring.


The microbiological tests at Peka Kroef are performed using leading technology equipment, such as incubators and heating chambers, supplied by the Binder. Here, the most important requirement for equipment is maximum temperature homogeneity in the test equipment interior. The Binder equipment with its patented technology guarantees homogenous air distribution and temperature stability even when the door is opened for the addition of new test samples. Any temperature fluctuations resulting from the intrinsic temperature of the samples and airflow from outside are balanced very rapidly.


In continuous operation, the incubators maintain a constant temperature of between 22°C and 55°C for specific applications. The incubators are used for a wide variety of applications such as warm storage of nutrient media, sample incubation, drying of food samples, or determining the presence of microorganisms with Durham test tubes. The various jobs are aligned in accordance with the programming of production processes. In this manner, any potential deviations, such as variations in raw material composition, can be detected and remedied with appropriate measures. As an additional safety measure, the Binder test chambers are regularly checked and recalibrated.


Circle no. 404


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