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Groundbreaking Metabolomics and Lipidomics Research Thermo Fisher Scientific, Inc
announced that the Human Nutrition Research (HNR) unit of the Medical Research Council (MRC) in Cambridge, UK, has selected the Thermo Scientific LTQ Orbitrap Velos, MALDI LTQ Orbitrap XL and Exactive mass spectrometers.
The Bioanalytical Sciences division at HNR, led by
Professor Dietrich Volmer, is a centre of excellence for developing innovative approaches to measure biomarkers for processes such as insulin resistance, energy expenditure and the availability of dietary nutrients within the body. Professor Volmer’s goal is to build a unique
population-based lipidomics platform allowing the application of studies of 10,000 or more samples. This platform will be used to establish biomarkers of nutritional intake, health and disease.
Professor Volmer recently received an award of £2 million from a translational medicine funding call of the MRC to establish the Cambridge Lipidomics Biomarker Research Initiative (CLBRI) within his research division. The MRC has recently purchased four mass spectrometers based on Orbitrap technology: an LTQ Orbitrap Velos™ biomarker discovery platform, a MALDI LTQ Orbitrap XL™ instrument as well as two Exactive™ benchtop liquid chromatography-mass spectrometry (LC-MS) systems. The Exactive instruments will be used in the high-throughput biomarker screening stages of the CLBRI projects, whereas the Orbitrap Velos will be applied to more detailed structural identification research such as those performed in biomarker qualification applications.
The MALDI LTQ Orbitrap XL provides unparalleled sensitivity for MSn analyses, a rapid scan rate, high mass accuracy and up to 100k resolving power. This enables the MRC to perform targeted analysis of phospholipids as well as unbiased profiling. The ability to apply both strategies in one experiment is an important step forward in the comprehensive analysis of the metabolome and the lipidome in particular. The Thermo Scientific Exactive is fast, easy-to-use and cost-effective to operate, making it an ideal instrument for non- experts and experienced mass spectrometry users. Using high-resolution mass spectrometry with instruments capable of measuring m/z ratios with relative mass measurement uncertainties of 1 ppm or less and sufficient scan speeds, researchers at the MRC are able to combine two strategies, allowing unbiased profiling of biological samples and targeted analysis of specific compounds in a single analysis without compromise.
Circle no. 348
A new fully automated, quantitative assay for beta-lactam antibiotics in milk has now been developed using online SPE- LC-MS/MS. A Spark Holland Symbiosis system for SPE-LC in combination with an Applied Biosystems-Sciex 3200 triple- quadrupole tandem mass spectrometer for MS/MS enables measurement of target compounds well below the Maximum Residue Limits set by the European Union. Ideal for food control and safety, this new developed method offers high sensitivity and accuracy of results, minimum sample pre- treatment, and for the first time uses an automated online SPE offering a high throughput analysis.
Conquering Mass Spectrometry Application Challenges
Waters Corporation introduced eight new MassLynx™ workflow enhancements at the 57th American Society of Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics, all designed to enhance laboratory productivity and expand mass spectrometry applications.
Brian Smith, Vice President, Mass Spectrometry Business Operations for the Waters Division, said: “These software innovations realise the full potential of mass spectrometry across our entire portfolio of LC/MS and GC/MS systems. MassLynx software continuously addresses new analytical challenges, and streamlines existing ones, through advanced data interrogation capabilities and simplified workflow processes.”
The POSI±IVE system™ is for high throughput screening of complex food safety, water quality and environmental samples by combining qualitative and quantitative processes into one. POSI±IVE takes Waters system solution paradigm to the next level by uniquely integrating chromatography, exact mass MS and informatics for screening applications. The BiopharmaLynx™ 1.2 increases confidence in peptide characterisation leveraging comprehensive exact mass UPLC/MSE data in biopharmaceutical applications.
Quanpedia, QCMonitor and TrendPlot, critical components of the Xevo™ TQ MS quantitative workflow solution software, are now available for a broader range of Waters mass spectrometers. Laboratories will cut sample analysis times and optimise resource utilisation to increase productivity with the latest version of OpenLynx™ Open Access. Clinical pathology laboratories will benefit from automated workflows for UPLC/MS/MS that include seamless HL7 LIMS integration.
MetaboLynx™ XS and MarkerLynx™ XS for accelerated metabolite identification and profiling are now available on all Waters MS Systems. Management of large proteomic data sets is accelerated with ProteinLynx Global SERVER™ (PLGS) 2.4 for nanoACQUITY 2D UPLC/MSE while data are now also interchangeable with PRIDE, Scaffold and mzML. DriftScope™ 2.1 features ion mobility spectrometry (IMS) peak detection, visualisation & comparison, together with collision cross section (CCS) determinations, for the original SYNAPT High Definition MS™ system and the new SYNAPT G2 HDMS system.
Circle no. 349 Ian Ransley to Head MS Developments at SGE
SGE Analytical Science has appointed Ian Ransley, PhD, as Director of the Mass Spectrometry (MS) Business Unit. Having joined the company as Product Manager for MS two years ago, Ian has followed many successful campaigns and was involved in that of the R&D 100 award winner MagneTof™ detector. Now overseeing the further development of the ETP electron multiplier business, Ian’s strong customer focus will continue to see him engage with our key partners around the world.
His experience, including product management roles for mass spectrometry products with Shimadzu and Applied Biosystems, is complimented by a strong research foundation, including a PhD in Surface Science from the University of Manchester, UK, fellowships at Cambridge and Warwick Universities and a Batchelor of Chemistry from Manchester University.
Stephen Tomisich, Managing Director of SGE commented “I am excited that Ian has taken on this position, he is well equipped to continue the strong growth shown by the ETP product line particularly as we look to broaden our reach in the industry”.
Circle no. 350 Setting a New
Benchmark for MS Ionicon is setting a new benchmark for on-line VOC monitoring/real-time mass spectrometry, for the first time touching a ppqv-range (parts per quadrillion) detection limit. Ionicon PTR-MS technology is trusted by scientists all over the world with good reason and is known for a market-leading single-digit pptv-level detection limit for most VOCs. With the
new PTR+SRI-MS featuring not only H3O+ but also O2+ and NO+ to chemically ionise a sample, Ionicon has taken this proven technology one step further.
For this new line of Ionicon PTR-MS instruments the sensitivity has been greatly enhanced for all three precursor ions yielding an unmatched detection limit in the several 100 ppqv-range that has been recorded using an off-the-shelve, Ionicon High- Sensitivity PTR-Quad-MS instrument with SRI capabilities. This important achievement is the outcome of Ionicon’s commitment to excellence in engineering and to a continuous technological evolution Ionicon shares with its PTR-MS customers.
Circle no. 352
Lina Kantiani from the Department of Environmental Chemistry of IDAEA-CSIC in Barcelona, Spain, described the fully automated assay for beta-lactam antibiotics in milk. Using a range of target compounds (6 penicillins and 4 cephalosporins), quantitation in milk ranged from 0.09ng/mL for ampicillin to 1.44ng/mL for penicillin G. Manual sample handling is greatly reduced involving only the addition of internal standard and centrifugation. Simultaneous analysis of all compounds takes just 10 minutes resulting in significantly increased throughput. The article concludes: “This analytical method presents an easy and cost- effective procedure for the identification and quantification of selected antibiotic compounds in raw milk samples and is a useful tool for the confirmatory analysis of these compounds in previous positively screened milk samples in routine food analysis.”
Antibiotic residues in food products can result in increased bacterial resistance against antibiotic medicines, carcinogenic and allergic responses in consumers and disturbances in milk culturing processes. Stringent legislation in Western countries requires the control and reduction of antibiotics residues in food and food producing animals in order to protect human health. Currently available test kits for screening for antibiotics in food products can lack specificity producing only semi-quantitative results, which may lead to false positives. Food control labs, therefore, tend to rely more and more on LC-MS/MS based assays for reliable confirmative measurements of samples after a prior screening test. Thorough sample clean up is required before samples can be processed by LC-MS/MS, and because existing methods use off- line sample preparation methods such as liquid-liquid extraction or solid phase extraction (SPE), such assays are labour intensive and suffer from low throughput.
Circle no. 351
MASS SPECTROSCOPY FOCUS
First Online SPE-LC-MS Assay for Antibiotics in Milk
LAB ASIA - NOVEMBER/DECEMBER 2009 - MASS SPECTROSCOPY FOCUS
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