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Expanded Immunomagnetic Separation Range for Shiga Toxin- Producing E. coli
Large Microplate Well Surface Area Promotes Efficient Cell Growth
Offering 24 high volume (3.1ml) wells in an industry standard 96-well plate footprint, the Krystal 24 from Porvair Science provides a large surface area per well, enabling unmatched cell growth.
Constructed in ultra-pure grade polystyrene, Porvair Krystal 24 microplates are tissue culture treated to ensure optimal cell growth and attachment. The special design of Krystal 24 plates makes them ideal for cell culture, cloning, viral titration, and cell fusion. Precisely manufactured to ANSI / SLAS microplate dimensions the affordable tissue culture treated Krystal 24 microplate is fully compatible with all commercially available plate readers, robotic sample processors and automated liquid handling systems.
Lab M has extended its immunomagnetic separation range with the launch of Captivate™ O121. This is for the detection and isolation of Escherichia coli O121, a non-O157 Shiga Toxin-Producing Escherichia coli (STEC) that is associated with foodborne infection and is one of the ‘big six’ organisms in meat products for which testing is required in the USA.
Although the most common serogroups of STEC in Europe and the USA are E. coli O157 or O26, the incidence of other serotypes is increasing. The six major non-O157 STECs identified in the USA are O26, O45, O103, O111, O121 and O145.
One of the challenges with E. coli O121 in particular is that it is not biochemically unique. Therefore it is difficult to isolate from other STEC strains using conventional culture methods, whereas the use of IMS supports the specific isolation of E. coli O121.
Immunomagnetic separation is now written into US Department of Agriculture (USDA) methodology for the detection and isolation of non-O157 Shiga Toxin-Producing (STEC) E. coli in meat products and so there is renewed emphasis in the use of this highly effective technique.
Lab M’s expertise in immunomagnetic separation dates back many years and Captivate™ is one of the company’s best selling product ranges around the world. As well as producing specific sets of antibody-coated paramagnetic particles for separating and concentrating targeted microorganisms, the company also offers a highly respected consultancy and custom coating service.
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The Krystal 24 plate edges and lids are designed to reduce evaporation, and the raised well rims reduce the risk of cross contamination. Porvair Krystal 24 lids are shaped to facilitate stacking. Krystal 24 plates and lids may be supplied sterile or non-sterile.
The unique Krystal 24 design also provides the convenience of direct measurements on bottom reading spectrophotometers and inverted microscopes. For sensitive fluorescence measurements the black Krystal 24 plate provides the all-absorbing background needed to minimise background interference. The opaque white Krystal plate maximises reflectivity enabling even weakly emitting luminescence assays to be routinely undertaken.
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Analytical Balances Combine Solid Performance and Simplicity
Adam Equipment, a world-class scale and balance manufacturer, offers PW analytical balances as an easy yet elegant choice for applications that require weighing to 0.1mg. Analytical balances can be costly but are necessary for hospital or research labs, where highly precise measurements are vital. Adam’s PW series of analytical balances delivers highly precise results at a value that is first in its class.
The PW features durable metal construction, standard internal motorised calibration and a large backlit dual display that shows text prompts to help the user set up the balance quickly. Highlighted tare keys stand out for accessibility, while other buttons operate set-up functions, turn the balance on or off, send data to a printer, calibrate and change modes and weighing units.
The RS-232 interface enables data transfer between the balance and printers or computers. This allows foolproof data recording and traceability, letting the operator focus on data analysis. Other valuable features include built-in applications for density, percentage, comparison and checkweighing, plus counting and dynamic or animal weighing. PW balances offer 16 weighing units, including a custom unit. With models from 120g to 250g and readabilities at 0.1mg, there is a PW analytical balance available for most laboratory applications.
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