Table 3. Retention time, asymmetry factor, and internal standards for method PFAS Peak No.
1 2 3 4 5 6 7 8 9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
1000
400 400 600 800
0 0 Figure 4. Typical calibration curves for PFAS Table 5. Precision and accuracy (n=6) of PFAS in fortified drinking water
Analyte PFBS
PFHxA
HFPO-DA PFHpA PFHxS
ADONA PFOA PFNA PFOS
9Cl-PF3ONS PFDA
PFUnA NMeFOSAA
11Cl-PF3OUdS NEtFOSAA PFDoA PFTrA PFTA
Fortified conc. (ng/L)
16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0 16.0
Mean recovery (%)
107 108 84.1 113 120 117 113 114 113 96.1 105 96.8 103 88.5 100 89.8 89.6 89.0
RSD (%)
3.3 2.3 7.5 2.7 3.4 2.5 2.5 2.9 4.5 4.1 3.2 5.0 5.2 5.5 9.9 4.4 3.8 4.8
Fortified conc. (ng/L)
80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0 80.0
Mean recovery (%)
98.3 106 88.6 117 123 121 119 118 117 103 111 103 110 97.1 104 97.3 95.8 98.1
RSD (%)
3.6 2.6 6.3 1.3 2.1 1.1 1.6 2.1 2.9 2.6 2.1 3.1 5.2 4.8 2.3 3.4 3.7 3.3
PFHxA
PFHxA r2
=0.999
Peak Name PFBS
PFHxA 13C2 13 -PFHxA HFPO-DA
C-HFPO-DA PFHpA
ADONA PFHxS PFOA
13C2 -PFOA
PFNA PFOS
13C4 -PFOS
9Cl-PF3ONS PFDA
13C2 d3 -PFDA
NMeFOSAA -NMeFOSAA PFUnA
d5
NEtFOSAA -NEtFOSAA
11Cl-PF3OUdS PFDoA PFTrDA PFTA
y=8.727x+0.000 0.999 1200
400 400 600 800 1000
0 µg/L
Retention Time (min) 4.56 6.56
6.56 7.16 7.16 8.37 8.57 8.58 9.65 9.66
10.66 10.70 10.70 11.16 11.50 11.50 11.96 11.97 12.19 12.34 12.35 12.50 12.78 13.27 13.70
9Cl-PF3ONS
9Cl-PF3 r2
=0.999 y=10.230x+0.000 ONS 0.999
Asymmetry Factor 1.09 1.01
0.96 0.84 0.84 1.01 1.12 0.95 1.06 0.98 0.99 1.03 1.04 1.16 1.03 0.95 1.08 1.05 1.00 0.93 1.10 1.05 1.07 1.01 0.94
1200
400 400 600 800 1000
0
25 50 75 100 120 0255075 100 120 025 50 75 100 120 µg/L
µg/L
Author Contact Details Thermo Fisher Scientific
Changling Qiu, Xin Zhang, Rahmat Ullah, Wei Chen, Yan Liu, • Sunnyvale, CA, USA • Email: Liu, Yan :
yan.liu@
thermofisher.com Qiu, Changling :
changling.qiu@thermofisher.com Zhang, Xin :
xin.zhang@thermofisher.com Ullah, Rahmat :
rahmat.ullah@thermofisher.com Chen, Wei :
wei.chen2@
thermofisher.com • Web:
www.thermofisher.com
PFTrDA
PFTrDA r2
=0.999
IS # ref
13C4 13C2 13C2 13C2 13C2 13C2 13C4 13C2 13C2
13C2 13C4
13C4 13C2 13C2
d3
-PFOS -PFOA -PFOA -PFOA -PFOA -PFOA -PFOS -PFOA -PFOA --
-PFOA -PFOS --
-PFOS -PFOA -PFOA --
-NMeFOSAA 13C2
13C2 d3
-PFOA -PFOA
-NMeFOSAA 13C4
13C2 13C2 13C2
-PFOS -PFOA -PFOA -PFOA
y=9.938x+0.000 0.999
Table 4. Calculated lowest concentration minimum reporting level and method detection limit results
Analyte PFBS
PFHxA
HFPO-DA PFHpA PFHxS
ADONA PFOA PFNA PFOS
9Cl-PF3ONS PFDA
PFUnA NMeFOSAA
11Cl-PF3OUdS NEtFOSAA PFDoA PFTrA PFTA
AutoTrace LCMRL (ng/L)a
0.30 0.63 2.2
0.38 0.68 0.20 0.59 0.23 0.89 1.1
0.72 1.2 1.5 2.1 3.5 1.6 2.6 2.5
AutoTrace DL (ng/L)b
0.59 0.44 1.8
0.42 0.49 0.30 0.41 0.38 1.2
0.77 0.75 0.79 1.1
0.62 2.5
0.99 0.71 0.86
References
1. U.S. EPA. Basic Information about Per- and Polyfluoroalkyl Substances (PFAS).
2. National Institute of Health, National Institute of Environmental Health Sciences, Polyfluoroalkyl Substances (PFAS).
3. U.S. EPA. Drinking Water Health Advisories for PFOA and PFOS.
4. U.S. EPA. Method 537.1, Detections of Selected Per- and Polyfluorinated Alkyl Substances in Drinking Water by Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS). Version 1.0, November 2018
5. Thermo Fisher Scientific, Determination of per- and polyfluorinated alkyl substances (PFAS) in drinking water using automated solid-phase extraction and LC-MS/MS, AN 73346-EN 0220S
Interested to learn more about Thermo Scientific LC-MS/MS with the automatic extraction AutoTrace 280 system, please go to:
thermofisher.com/AutoTrace280-pfas-in-drinking-water
AET ANNUAL BUYERS’ GUIDE 2020
WWW.ENVIROTECH-ONLINE.COM
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