12 ENVIRONMENTAL LABORATORY New technology sheds light on Salmonella and Listeria


Nemis Technologies Ltd is excited to announce the recent publication of its research work on the innovative, ultra-sensitive chemiluminescence-based technology for the detection of Salmonella and Listeria monocytogenes has been published in the prestigious journal Angewandte Chemie. In the journal, a team of scientists from Tel Aviv University (Professor Doron Shabat), The Zürich University of Applied Sciences (Professor Lars Fieseler), Nemis Technologies AG and Biosynth AG have introduced a new, ultrasensitive, chemiluminescence-based method for the direct detection of Salmonella and Listeria monocytogenes. The study is titled ’Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small-molecule Chemiluminescence Probes’.


The method is based on chemiluminescence, the emission of light resulting from a chemical process. The simplicity of the tests allows for both enrichment of the bacteria and their detection in a test tube, with no further sample preparation and therefore, no containment laboratory is required. The chemiluminescence probes have proven to be about 600 times more sensitive than conventional fl uorescence probes. The success of this technique is due to specially developed molecular probes combining a luminescent substance (a phenoxy-dioxetane) with a ‘trigger’. In this form the probe does not light up. The trigger is tailored to the bacteria to be detected; it is recognised by a specifi c enzyme produced by the pathogen - a special esterase in the case of salmonella and a special phospholipase C for listeria - that splits it from the luminescent part.


This enzyme initiates a chemical reaction that causes the luminescent molecule to split and emit a very intense green light. Tests with various bacteria demonstrated that the probe tailored to the Listeria test only reacts to Listeria monocytogenes, not to other, non-pathogenic, strains of Listeria. The sensitivity of the test allows fi rst results after six to eight hours and same day detection can be achieved even with dried bacteria from food industry surface materials, such as stainless steel. Arnaud Muller, the company’s CEO, stated: “Excited by these fi ndings; a great teamwork in developing our innovative technology. We are confi dent that our new method will be used more broadly to develop specifi c chemiluminescence probes relevant for the detection of a wide range of bacteria and point-of-care diagnostics. Additional detection kits are currently under development at Nemis.“ More information online: ilmt.co/PL/KJAw


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49758pr@reply-direct.com “ Environmentally friendly cars will soon cease to be an option…they will become a necessity ” — Fujio Cho No frills HPLC autosampler


Mercury, Arsenic and Selenium Determinations for On-line Wastewater Applications – Step into the Future


P S Analytical is delighted to showcase the new trace metal monitoring analysers, particularly for the toxic mercury (Hg), arsenic (As) and selenium (Se) elements in wastewater streams, based on our ultra-sensitive and market leading Atomic Fluorescence Spectroscopy (AFS) technology.


We are excited to bring some real game changing advances to this application area with the launch of our new third generation, online systems for wastewater monitoring.


The systems are particularly useful for waste streams from coal fi red power stations, semiconductor fabrication plants and other similar industries. The systems also fi nd use for drinking water applications in parts of the world where these elements are present in the ground water.


Detection performance and lack of interferences have always been forefront in our design approach. This continues with this instrument release, which also benefi ts from syringe-based reagent delivery and, for mercury determinations, employs a gold amalgamation trap as an integral part the detector.


Moving away from the continuous fl owing reagent streams, to a discrete approach reduces reagent consumption and cost signifi cantly and improves chemical reaction conditions to allow better sensitivities with reduced interferences.


The new design incorporates onboard UV oxidative heating for sample pre-treatment, fully automated calibrations and multiple stream selection including manual grab samples.


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delivers what you truly need IF your HPLC routine calling for automation, look no further than the HT1500L: an autosampler specifi cally designed for HPLC, fully equipped with all the powerful features any HPLC technician would naturally choose.


HT15000L is the new HPLC autosampler from HTA that offers the best balance of quality and affordability and that is perfectly compatible with any HPLC system.


Unlike LC-MS, nanoLC and UHPLC systems, the HPLC requires few, essential features: excellent analytical performance, good sample capacity, and of course, uncompromised reliability, robustness and durability. HTA has showered the HT1500L with all these essential features, while eliminating anything unnecessary to keep costs as a minimum.


No frills but even no compromise. Despite the competitive price, HT1500L has a strong commitment to quality: the materials have been picked up to provide longevity; the up- to-date technologies offer reliability that deserve a modern instrument while the design attests our care in details.


HT1500L holds multiple racks, each of them removable, to allow continuous sample feeding and more convenient operations. The on-board solvent reservoir allows the needle and the whole fl owpath cleaning before processing the next sample.


HT1500L comes with HTA Autosampler Manager, HTA complementary software for autosampler programming. After setting the method once, the operator just needs to load samples and presses START: all the samples in the racks will be automatically processed. Seamless integration can also be experienced with the currently used Chromatography Data System.


HT1500L can actually become your workhorse, extremely reliable and straight to the point. More information online: ilmt.co/PL/rkR3


Know your ions - overcoming the limits


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of HPLC with ion chromatography What’s the difference between High Pressure Liquid Chromatography (HPLC) and Ion Chromatography (IC)? What are the capabilities and limitations of each technique? Knowing these differences can actually make a huge difference. A white paper from Metrohm discusses ‘When HPLC fails’ compares the two methods systematically focusing on the strengths of ion chromatography.


HPLC is one of the most powerful analytical techniques in the modern laboratory. From research to manufacturing to legal, it is widely used in many sectors. However, there are some inherent limitations. Ionic and polar compounds pose a challenge. So do organic acids. Moreover, some analytes, such as fl uoride and chloride, are not active in the UV range, making it impossible to determine them with a UV detector, the detection technique most commonly used with HPLC. Ion chromatography (IC), on the other hand, is ideal for the separation and detection of ions and polar molecules, both organic and inorganic. This makes IC the ideal extension of HPLC to increase the analytical capabilities of the modern laboratory greatly.


Over the last 30+ years, Metrohm has made Ion Chromatography a powerful tool for both research and routine analysis. Laboratories in the many sectors in particular benefi t not only from the method itself but from many automated inline sample preparation and injection techniques. Samples with a high load of particles (e.g., wastewater), solid and even gaseous samples can be analysed fully automatically down to the µg/L range with superior reproducibility. Hundreds of IC applications are available free of charge from the Metrohm Application Finder.


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