Health – Aberdeen University
recovering fish. SFIRC (Aberdeen) in collabora- tion with CEFAS-Weymouth, has used state- of-the-art laser capture techniques to purify parasite stages from kidney tissues in order to develop a fish-specific parasite gene library. Most recently we have used genome sequenc- ing technologies to develop high throughput parasite gene datasets from both hosts. Comput- er based analysis has enabled us to determine which parasite genes appear to be host-specific/ dominant and those which are expressed in both hosts. We have recently obtained conclusive evidence
showing the dominance of several parasite genes in trout kidney over parasite stages in bryozoans, one of which is a potential surface expressed molecule captured from our ELI studies. In a new Aberdeen based study, to complement ELI we (Holland, Secombes and PhD student Marc Faber) will be developing a high throughput
26
automated pipeline to shortlist more antigens for testing as suitable vaccine candidates. Knowledge of the host specificity of parasite genes will be an important criterion in this pipeline, along with a number of computer based tools to identify the most important antigens to take forward.
This study will also, for the first time in PKD
research, characterise the host response to invasive parasite stages in gill tissue in order to understand how T. bryosalmonae avoids the fish immune system at this early stage, and to develop potential immune therapies to combat severe kidney pathology during clinical PKD. This work will be done as part of a Horizon 2020 (EC)-funded Europe-wide research con- sortium called ‘ParafishControl- Advanced Tools and Research Strategies for Parasite Control in European farmed fish’. Overall, the last few years have seen great
advances in our ability to sequence and analyse genomes and expressed genes, and together with methods such as ELI that allow screening of large numbers of vaccine candidates, development of parasite vaccines for fish no longer seems an intractable goal. By Jason W Holland, Sohye Yoon, Bar- tolomeo Gorgoglione and Christopher J Secombes, Scottish Fish Immunology Research Centre, University of Aberdeen
For further reading see: B Gorgoglione, T Wang, CJ Secombes, JW Holland, 2013. Immune gene ex- pression profiling of Proliferative Kidney Disease in rainbow trout Oncorhynchus mykiss reveals a dom- inance of anti-inflammatory, antibody and T helper cell-like activities. Veterinary Research Vol. 44, 55. B Okamura, A Gruhl, JL Bartholomew, 2015. Myxozoan Evolution, Ecology and Development, 1st ed. Springer. FF
www.fishfarmer-magazine.com
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68