» RAMAN
»
during dissolution” at the University of Twente. This work includes CARS/ SRS imaging of pharmaceutical formulations. His research interests include spectroscopy for pharmaceutical applications, and pharmaceutical formulation science.
Antti Isomäki received his Ph.D. in physics from Tampere University of Technology, Tampere, Finland in 2007. From 2008 to 2012 he worked as a postdoctoral researcher at the Technical University of Denmark with his focus on the development of multimodal imaging techniques for
biomedical applications. He is currently working as a multiphoton imaging specialist at the Biomedicum Imaging Unit, University of Helsinki.
Clare Strachan is an Assistant Professor (tenure track) at the University of Helsinki, Finland. Her main research involves pharmaceutical applications of vibrational spectroscopy and nonlinear optical imaging, as well as formulation of poorly water soluble drugs. She completed
a Ph.D. in 2005 at the University of Otago, New Zealand, and has authored approximately eighty international peer-reviewed journal publications. In 2013 she was appointed an Honorary Associate Professor at the School of Pharmacy, University of Otago.
Corresponding author – e-mail:
clare.strachan@
helsinki.fi Tel: +358 9 191 59736
References 1.
Figure 4. Penetration of different compounds into mouse skin using SRS microscopy, (a) Raman spectra of dimethyl sulfoxide (DMSO, green), retinoic acid (blue), and lipids in mouse skin (red), (b) top view of the penetrated DMSO
(green), (c) stimulated Raman loss (SRL) DMSO depth profile through the line in (b), (d) simultaneous SRL two color image of DMSO (green) and lipid (red) at subcutaneous layer at a depth of about 65 μm though the lower line in (c), (e) top
view of the penetrated retinoic acid (blue), and (f) retinoic acid depth profile through the line in (e). Reproduced from [24] with permission of the American Association for the Advancement of Science.
diverse applications is expected to dramatically increase, in both industry and academia.
Author Biographies
Andrew Fussell graduated from the University of Otago in 2008 with a bachelor in pharmacy with honors and registered as a pharmacist in 2009. In 2010, he completed his postgraduate certifi cate in pharmacy. He is currently working on his Ph.D. titled “In situ imaging of dosage forms
62 | | September/October 2013 - 15TH ANNIVERSARY ISSUE 2. 3. 4. 5. 6. 7.
Helmchen, F. and W. Denk, Deep tissue two-photon microscopy. Nature Methods, 2005. 2(12): p. 932-940.
Schenke-Layland, K., et al., Two-photon microscopes and in vivo multiphoton tomographs - Powerful diagnostic tools for tissue engineering and drug delivery. Advanced Drug Delivery Reviews, 2006. 58(7): p. 878-896.
Pautot, S., et al., Spontaneous formation of lipid structures at oil/water lipid interfaces. Langmuir, 2003. 19(24): p. 10281-10287.
Day, J.P.R., et al., Label-free imaging of lipophilic bioactive molecules during lipid digestion by multiplex coherent anti-stokes raman scattering microspectroscopy. Journal of the American Chemical Society, 2010. 132(24): p. 8433-8439.
Kang, E., et al., Paclitaxel distribution in poly(ethylene glycol)/poly(lactide-co-glycolic acid) blends and its release visualized by coherent anti-Stokes Raman scattering microscopy. Journal of Controlled Release, 2007. 122(3): p. 261-268.
Kang, E., et al., Application of coherent anti-stokes Raman scattering microscopy to image the changes in a paclitaxel–poly(styrene-b-isobutylene-b-styrene) matrix pre- and post-drug elution. Journal of Biomedical Materials Research Part A, 2008. 87A(4): p. 913-920.
Kang, E.N., et al., In situ visualization of paclitaxel distribution and release by coherent anti- stokes Raman scattering microscopy. Analytical Chemistry, 2006. 78(23): p. 8036-8043.
8. Windbergs, M., et al., Chemical imaging of oral solid dosage forms and changes upon dissolution using coherent anti-Stokes Raman scattering microscopy Analytical Chemistry, 2009. 81(6): p. 2085–2091.
9. 10.
Jurna, M., et al., Coherent anti-Stokes Raman scattering microscopy to monitor drug dissolution in diff erent oral pharmaceutical tablets. Journal of Innovative Optical Health Sciences, 2009. 2(1): p. 37-43.
Fussell, A., et al., In situ dissolution analysis using coherent anti-Stokes Raman scattering (CARS) and hyperspectral CARS microscopy. European Journal of Pharmaceutics and Biopharmaceutics, (0).
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152 |
Page 153 |
Page 154 |
Page 155 |
Page 156