46 Highly Efficient Exosome Isolation and Purification Tools


AMSBIO is a knowledgeable supplier of products dedicated to exosome research that includes high quality reagents and kits that are quick, affordable and easy to use.


The latest addition to this portfolio is a new range of tools for isolating and purifying overall or specific exosome sub-populations from small volumes of sample in an efficient, faster and cheaper way.


For applications that require total exosome isolation from biological fluids including plasma, serum and urine as well as cell culture media, AMSBIO has launched ExoPure™ Reagent - a fast and convenient one-step method based on chemical precipitation.


AMSBIO’s new ExoPure™ Isolation kit offers a fast and convenient method of exosome isolation and purification at high yields from body fluids including Cerebrospinal, Amniotic, Inflammatory, Lymph, Breast milk, Saliva, Gastrointestinal and Broncho alveolar lavage fluids. Using this kit enables researchers to more reliably produce highly pure exosomes compared to using exosome precipitation methods.


ExoPure™ Immunoplates are 96-well microplates, covalently pre-coated with specific exosome-binding antibodies to allow exosome capture and isolation from sources including cell supernatant, human plasma, serum, urine and saliva. These high-performance plates are available in transparent, white and black versions tailored to suit the downstream detection approach employed (colorimetry, luminometry or fluorimetry methods respectively).


AMSBIO new range of ExoPure™ Size Exclusion Columns (SEC) are designed for isolating extracellular vesicles in a fast and easy way. These columns are available in two different sizes of column to enable optimised extracellular vesicle purification from biofluids (plasma, serum, urine) and cell media and for different sample volumes.


More information online: ilmt.co/PL/mxOQ 55990pr@reply-direct.com


New Autoclave Series Offers Advanced CFR 21 Part 11 Solution


The criteria defined in FDA CFR 21 Part 11 ensures improved efficiency with automated, paperless and tamper-proof digital processing of process flows and audit trail data. With the ‘Advanced CFR 21 Part 11 solution’ Systec’s HX series of autoclaves supports documentation according to FDA CFR Part 11, without any additional software or hardware.


Process data can be exported and saved using the Advanced CFR 21 Part 11 solution of the new Systec HX series. Systec’s innovative solution also provides: exporting data as PDF or CSV files that are digitally signed by the autoclave according to the X.509 standard; exported via USB port or network interface (automatic export to FTP, SFTP and FTPS server only with ‘Save to Folder’ option); security function to automatically invalidate data if a manipulation attempt was detected; advanced user account control for up to 100 customisable groups and 100 users; AuditTrail for the chronological documentation of detailed system and process activities which cannot be altered.


All exported information (PDF and CSV files, AuditTrail logs) is documented in accordance with CFR 21 Part 11. They are unalterable and tamper- proof. The data can be unambiguously assigned to its corresponding autoclave, since they are electronically signed by the autoclave and immediately marked as invalid if a manipulation attempt is detected. Thus, the correct handling of the autoclave can be proven to supervisory authorities.


The Systec HX series software package is pre-installed and runs independently of third-party hardware. Therefore, external PCs or storage media are only required to export the data as signed PDF or CSV files. AuditTrail information can also be displayed on the autoclave’s integrated touch display and exported to a USB flash drive.


The Advanced CFR 21 Part 11 solution for Systec’s HX series of autoclaves optimises the digital documentation process based on the FDA CFR Part 11 regulations; this reduces hardware and software costs and ensures total compliance with international standards. The hardware and software components of the autoclaves can also be adapted to the requirements of different industries by using additional options.


Download a short overview about the CFR 21 Part 11 solution: ilmt.co/PL/Qorp More information online: ilmt.co/PL/gO6l


56292pr@reply-direct.com


Daisy-Chaining Photo- and Thermal Chemistry


Uniqsis reports how researchers at the School of Chemistry, University of Bristol have successfully prepared bicyclic lactams in high overall yield and excellent purity, under flow through conditions, using a photochemical [2+2] cycloaddition followed by a thermal electrocyclic cascade.


By moving the photochemistry, performed using a PhotoSyn™ LED photoreactor, from ultraviolet (UV) to visible, the Bristol researchers were able to make their [2+2] ring closure much more energy-efficient. Integrating this with a FlowSyn™ Continuous Flow Reactor they were also able to apply automated high-temperature flow chemistry to the thermal cascade step facilitating safe and reliable scale-up with short reaction times.


Using this experimental set-up, the researchers were able to demonstrate a unique example of a photothermal daisy-chained process combining a visible-light-mediated photochemical initiation and a thermal cascade reaction as a fully continuous process yielding 20 grams/hour. This process was facilitated by the ability to employ acetonitrile as solvent at high temperature under pressurised conditions and the precise control of residence time and temperature in the flow reactor which enabled the isolation of a reactive diene intermediate on a scale and yield not achievable under batch conditions.


For further information on the research work undertaken by the University of Bristol please download application note 32: ilmt.co/PL/PY5j More information online: ilmt.co/PL/XkKZ


56233pr@reply-direct.com Determination of Implant Ceramics Using Dynamic Light Scattering


The Bl-200SM research goniometer system from Testa Analytical Solutions eK provides unmatched performance and operational versatility for Static Light Scattering (SLS) and Dynamic Light Scattering (DLS) studies.


Researchers from Eötvös Loránd University, the oldest and largest university in Hungary, have used a BI-200SM in Dynamic Light Scattering mode to determine the aggregate size of powders used in producing sintered bulk calcium silicate ceramics.


These sol-gel synthesised ceramic powders were then studied to determine their properties in relation with biomedical applications. Using light scattering data obtained using the BI-200SM research goniometer system the researchers were able to identify the best bulk ceramics with good mechanical strength, low solubility and similar porosity to bone, allowing them to be used as long-term implants.


The Bl-200SM goniometer system is a precision research grade instrument designed for exacting light scattering measurements. Based upon a special turntable with precision ball bearings and stepping motor, the Bl-200SM’s modular, automated design and quality construction guarantee precise measurements due to the wobble-free movement of the detector. Designed and built for demanding research experiments the BI-200SM can measure light scattering over a wide angular range (8° to 155° with 25 mm cells) and offers fine adjustment of measurement angles to 0.01° directly using a large, fine-control knob or PC-driven Motor control. Fine-screw vertical adjustment makes centre of rotation measurement easier when aligning cells. Precise repeatable data is ensured by automated heating and cooling of the sample cell using and standard external recirculating system. Field proven in hundreds of labs around the world the BI-200SM light scattering goniometer system is ideal for even the most demanding macromolecular studies and submicron particle sizing applications.


More information online: ilmt.co/PL/QoZg 56408pr@reply-direct.com


LABMATE UK & IRELAND - NOVEMBER 2021


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56