Luminescence, UV & Microplate Readers
Rapid Protein Labelling Kit Launched
Fluidic Analytics Ltd has launched a time-saving and affordable protein labelling kit - fluidiphore rapid amine 503. Absorbing at 503 nm, the new kit can be used with a multitude of techniques including the company’s groundbreaking Fluidity One-W instrument for protein interaction analysis.
Fluorescent labelling of proteins such as antibodies, DNA and lipids is widely used to investigate and better understand protein structure and function. There are various fluorescent dyes available on the market, most of which require a long labelling process over several hours and a purification step to remove excess dye.
Fluidic Analytics’ fluidiphore labelling kit is extremely rapid, taking just 30 minutes to bind proteins rather than four hours to overnight, as is required by other kits. In addition, the fluidiphore kit uniquely does not require a purification step. This is due to a clever ‘triple-lock’ against background effects. Firstly, the absorbance of the dye shifts from 612 nm (free) to 503 nm when conjugated, meaning that unbound dye will not fluoresce at the same excitation wavelength. Quantum yield (the ratio of fluorescence emission to absorption) also increases upon conjugation meaning that unbound dye will have a much weaker signal than bound. Thirdly, any unused dye hydrolyses during the labelling process becoming inactive and unable to bind, therefore preventing the shift in absorbance and increase in quantum yield.
In addition, there is also a useful observable colour change from blue to red/yellow which confirms that labelling is taking place, giving researchers additional confidence in the process.
The fluidiphore rapid amine 503 labelling kit is ideal for use with Fluidic Analytics’ Fluidity One-W instrument to study proteins such as membrane proteins, multi-protein complexes, and intrinsically disordered proteins. The Fluidity One-W enables researchers to accurately assess on-target protein interactions in solution, even in complex, unpurified backgrounds such as crude lysates or blood plasma. The instrument reports absolute size of bound and unbound species, stoichiometry and the nature of binding can be assessed at the same time as binding affinity, giving a complete picture of binding events.
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Live Cell Plate Reader with Real-time Image Cytometry
The innovative Spark® Cyto plate reader from Tecan is a unique system is the first live cell plate reader to offer real-time detection and analysis of biological, chemical and physical events, capturing the maximum amount of data from every well, at the same time and under the same conditions. Building on the success of the original Spark platform, it combines the flexibility of a high-end multimode plate reader with whole well imaging and comprehensive environmental control for cell-based assays.
Spark Cyto uses top-of-the-range camera components and a patent-pending LED autofocus system to provide real-time data acquisition and analysis - for 6- to 384-well formats - ensuring that no key event is ever missed. It allows qualitative and quantitative information to be integrated into unique multiparametric data sets, delivering meaningful insights faster than ever before. With three magnification levels and four acquisition channels, it enables entire cell populations to be investigated by capturing the whole well area of 96- or 384-well microplates in just one image, without tiling or distortion.
Powerful SparkControl™ and Image Analyzer™ software give the operator complete control of all experimental parameters, with predefined methods for common cytometry applications. Together with user-programmable advanced features - such as Real Time Experimental Control (REC™) for automatic performance of kinetic experiments - this unlocks new possibilities for cell-based research.
Dr Fabio Gasparri, Head of Cell Assays at Nerviano Medical Sciences and an early user of Spark Cyto, commented: “I was impressed by the system’s automated cell incubation and analysis capabilities. Cells were growing with a similar doubling times, and even more homogeneously, compared to a dedicated cell
incubator approach. The whole well imaging capability for 96-well plates allowed reproducible analysis in real time.”
Dr Valery Bochkov, Professor of Molecular Pharmaceutics at the University of Graz, Austria, agreed: “Using the Spark Cyto for cell imaging, enzymatic tests and immunoassays, we were impressed by how intuitive and easy to use the software is. Our diploma and PhD students instantly learned how to work with Spark Cyto and obtain high quality results.” More information online:
ilmt.co/PL/v0LM
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New UV Ring Light for Stereo Microscopy Launched
The new SCHOTT® VisiLED UV Ring Light for stereo microscopes combines classic bright-field illumination with UV illumination. It is the only segment ring light on the market in which white-light and UV-LEDs are alternately installed in eight segments. This LED arrangement allows objects to be examined from the same illumination angle, which significantly improves the ability to compare and reproduce resulting images. While the homogenous white light displays samples in natural colour, UV excitation reveals previously invisible parts of the sample through fluorescence. The SCHOTT MC 1100 Controller (available for separate purchase) makes it easy to switch between the two illumination modes.
The UV Ring Light for stereo microscopes is used in applications such as forensic document examination, crack detection or examination of paints and varnishes. With fluorescent markers and UV light, previously invisible details can be made visible. A UV protection filter in the observation path ensures that the user can look safely at the sample through the eyepieces and clean operation of the microscope camera.
“We were increasingly asked by customers for UV illumination that is safe and easy to use,” said Dr Christian Zawar, Senior Sales Manager at SCHOTT Lighting and Imaging. “In addition, the user always needs a normal bright field illumination on the microscope. Our new VisiLED UV ring light combines both of these capabilities in a unique way.”
The VisiLED UV Ring Light contains UV-A LEDs that emit wavelengths between 340 and 420 nm, with a central wavelength of 375 nm. The new UV ring light offers an illuminance of 100 klx in bright-field mode and 160 W/m² in UV-illumination mode. Each ring light comes equipped with the SCHOTT GG435 UV Protection Filter. Although the segment ring light belongs to risk class 3 according to IEC62471, the filter ensures maximum safety for the eyes when looking into the eyepieces.
More information online:
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