Luminescence, UV & Microplate Readers


High-Throughput Screening using Luciferase Reporter Cell Lines


Amsbio reports on the positive customer feedback received for their One-Step Luciferase Assay kit recently introduced for quantitation of firefly luciferase activity in mammalian cell culture.


Dr Maja Petkovic, Custom Services Business Unit Manager at Amsbio said: “Initial uptake of this new kit has been strong particularly for high throughput screening applications. Our customers have appreciated that the kit offers not just high sensitivity but also the convenience of a homogeneous, single step protocol. In a recent study, researchers at Excellerate Bioscience were able to quantify agonist and antagonist in vitro pharmacological parameters in a luciferase reporter gene assay using our One-Step Luciferase assay system.”


Luciferase is the general term given to a class of oxidative enzymes that catalyse reactions that give off bioluminescent light. Biological researchers can take advantage of this reaction and use it as a readout for processes where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase.


The One-Step Luciferase Assay kit combines proprietary reagent buffer and reagent substrate components to form a working solution that contains all the necessary components for simple cell lysis and luciferase quantitation. Designed for flexibility - the kit works well with a variety of common media, delivers a signal output that is stable for more than two hours and does not require a luminometer with injectors.


52314pr@reply-direct.com Optimal Filter Performance for Quantitative Polymerase Chain Reaction


Optical filters play a crucial role in detecting the corona pathogen SARSCoV2. With the expanded Ultra series, Laser Components now offers optical filters that were specifically developed for real-time qPCR tests for the SARS-CoV-2 virus. Patient samples can thus be analysed faster and more efficiently.


The polymerase chain reaction (PCR) is a proven method in which the inactive genetic material of viruses is exponentially multiplied so that it can finally be detected in the laboratory. In quantitative real-time PCR, additional fluorescent dyes are added to the reaction, which become active at a specific point in time during the chain reaction. This makes both quantitative analysis and detection possible. The performance of this method depends heavily on how accurately the fluorescence wavelength is detected. Bandpass filters that suppress all other wavelengths ensure this.


Blocking that is greater than OD6 results in bandpass filters that have a transmission in the pass band of more than 95% and a medium wavelength tolerance of less than ± 0.3% at the required wavelength to increase qPCR efficiency. These filter sets are optimally adapted for use with fluorescent dyes such as FAM, Cy5, HEX, JOE, ROX, TexasRed, and TAMRA.


Not only manufacturers of medical PCR instruments will be able to benefit from them. More information online: ilmt.co/PL/96mn


Operator-free GMP-Compliant Cell Sorting System Introduced


With its new MACS® GMP Tyto® Cartridge, Miltenyi Biotec opens new possibilities for fluorescence-based, GMP-compliant cell sorting. Specifically developed for the MACSQuant® Tyto® Cell Sorter, the launch of the cartridge makes Miltenyi Biotec the first company ever to offer GMP-compliant multiparametric, fluorescence-based cell sorting under safe and sterile conditions.


To help researchers meet the requirements of regulatory authorities for cell manufacturing protocols, every MACS® GMP Tyto® Cartridge comes with a batch-specific certificate of analysis (CoA), a TSE risk statement, and a product information file (PIF). PIFs, provided for all MACS® GMP Products, have proven very useful in the discussions with regulatory authorities, as they give detailed information on raw materials, QC release testing, sterility testing, biocompatibility, endotoxin levels, packaging, transport, and shelf life.


The MACSQuant® Tyto® Cell Sorter with its patented microchip-based technology allows for high-speed, multiparameter cell sorting in a fully closed system. Cells remain inside the closed cartridge at all times during the sorting process, and no sheath fluid is present in the system. Miltenyi Biotec’s range of MACS® GMP Fluorescent Antibodies and MACS® GMP Tyto® Running Buffer complement the system to make sure that cells only come in contact with GMP-compliant products.


Due to the closed design of the cartridge, cell sorting using the MACSQuant® Tyto® Cell Sorter provides full operator and sample safety. The easy ‘plug-and-play’ format of the instrument, combined with automated cartridge alignment and operator-free sorting, makes cell sorting accessible to any lab professional, without intensive training.


More information online: ilmt.co/PL/6PA3 52595pr@reply-direct.com 52594pr@reply-direct.com


Next-generation benchtop Energy Dispersive X-Ray Fluorescence (EDXRF) spectrometer Bruker’s S2 PUMATM Series 2 is equipped with HighSenseTM


Please watch the recorded webinar now available on Bruker’s website.


technology for increases in throughput by about a factor of 3. Bruker’s SPECTRA.ELEMENTSTM


software comes with enhanced features and faster algorithms, leading to ~40% shorter evaluation times. The S2 PUMA Series 2 supports elemental analysis applications from cement, steel, mining and petrochemical, to food analysis and pharma QC. The new mapping stage also extends its applications into semiconductors and coatings, where spatial resolution is required.


51924pr@reply-direct.com


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