27 2. Column:


a. A mismatched solvent / stationary phase polarity can be amended by adjusting the solvent or stationary phase to allow wetting.


b. If the sample loading capacity is exceeded, inject less sample onto the column by either diluting the sample, using a split injection or reduce the injection volume.


These systems, like all chromatographic techniques, have a list of quite common problems seen by analysts. Missing peaks,


tailing, carryover, fronting, inconsistent peak area, baseline noise, to name a few. If any of these terms seem familiar, don’t worry, you’re not alone. These issues have been around ever since Martin and James invented the technique in 1952!


A free trouble shooting guide by Shimadzu discusses these common problems and a list of helpful actions that can be taken. It offers guidance on how to approach certain problems in a methodical, scientific manner. Patience is required and don’t be


disheartened if your first course of action doesn’t resolve the issue. Remember that the most obvious answers are usually the correct ones, and there is nothing wrong with checking the basics first.


Finally, if you do have a problem please remember to call your instrument provider. Ultimately, they are here to help, and all want to see instruments up and running as much as you.


The guide is available from: www.shimadzu. co.uk/form/gc-poster


Glass Autosampler Vials Virtually Eliminate Surface Activity


Reduced Surface Activity (RSA™) glass autosampler vials are not coated vials and virtually eliminate the adsorption of basic compounds found with all other glass vials. The manufacturing process of RSA™ vials produces vials without surface activity such as basic compound adsorption. Unlike ordinary glass vials, these vials will not produce a pH change with aqueous diluent over time in the vial and with minimised surface metals, it is excellent for LCMS in that it does not contribute to sodium adducts.


Laboratories testing low abundance analytes such as low dosage form pharmaceuticals, unknown unknowns and natural products will see the greatest benefit even though all labs will find these vials to have value when repeating runs or investigations must be avoided.


The vials are available from Microsolv in 12x32mm clear and amber with volumes of 2ml, 1.5ml and 300ul. More information online: ilmt.co/PL/ZJGm


Monolithic Chromatography wand its Modern Applications


High-performance liquid chromatography has become the predominant global separation technique over the past four decades. However, the recent development of an alternative column packing material - a one- piece porous solid known as a ‘monolith’ - has transformed the field. Monoliths have attracted a large amount of interest due to the enhanced speed and thoroughness with which they can separate complex mixtures of biological molecules.


Monolithic Chromatography and its Modern Applications is the book for any seasoned scientist already using monolithic columns, as well as those novices who are new to the area and would like to know more about this technique. It’s the ideal resource for all analytical laboratories thinking of improving their separation protocols. In this book, global experts comprehensively describe this developing field, with part one covering the synthesis and characterisation of the columns and part two discussing their numerous applications.


More information online: ilmt.co/PL/YpPB


RSA™


Autosampler Vials for Not All Vials Are Created Equally & Only RSA™


better chromatography data. Vials:


will Prevent pH Changes in the Vial Beware of ordinary borosilicate glass vials, they have variable surface chemistries.


…and are Great for


Bio-Active Compounds Prevent hydrolysis in your vial due to glass with reduced surface silanols.


…and will Minimize


Sample Adsorption Insure quantitation precision run to run for low abundance basic compounds.


…and will Prevent Adducts Seen in


LCMS from the Vials Minimal surface metals.


…and are the Cleanest


Vials on the Market Prevent spurious peaks from ordinary glass vials that contain residual manufacturing agents.


…and are made with


Superior Dimensional Control Protect your sample needle and instruments.


EXCLUSIVELY BY: TRY THEM TODAY! www.rsa-glass.com


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