Chromatography 13 SFC/UHPLC Switching System Combines Improved Efficiency with Reduced Cost of Acquisition
Shimadzu’s Nexera UC/s (SFC/UHPLC switching system) allows measurements either by liquid chromatography (LC) or supercritical fluid chromatography (SFC) on a single system. LC and SFC offer very different selectivity for analytes of interest, so an increased range of compounds can be analysed. Switching between SFC and LC permits rapid screening for optimum separation conditions resulting in improved analytical efficiency. Existing Shimadzu (U)HPLC systems can be upgraded easily to build a Nexera UC/s, offering a full SFC/UHPLC setup while saving acquisition cost as well as lab space.
Liquid chromatographs are widely used in drug discovery, food safety analysis, environmental control and many other application areas. However, separation of components with identical physical and chemical properties, such as optical isomers, is challenging, and method development can take a very long time.
Supercritical fluid chromatography (SFC) using a mobile phase having both liquid- and gas-like properties offers an interesting alternative in selectivity and separation behaviour. Especially for separation of chiral compounds, SFC has frequently been shown to be the method of choice. It can also generate up to 5 times the linear velocity of RP-LC, while reducing the amount of organic solvent consumption.
Each technique has its advantages, and superior separation results can quickly be obtained by screening with both SFC and (U)HPLC separation methods, using the same instrument to improve efficiency and increase productivity.
With Nexera UC/s, components that co-elute using LC can easily be re-tested using SFC. A hybrid system enables most efficient method screening using the two orthogonal techniques in one sequence.
Since the Nexera UC/s provides two separation modes on a single system, it requires less space than two dedicated systems and also reduces the initial purchase price. A newly-released kit to upgrade an existing Nexera UHPLC system to a Nexera UC/s further decreases the investment cost for the additional SFC system.
Using the dedicated Method Scouting Solution software, screening sequences using (U)HPLC or SFC separation methods are created automatically. The process of switching between SFC and (U)HPLC methodology can be accomplished easily in just a few minutes, providing stable results with excellent reproducibility even when switching between separation modes in one batch.
The Nexera UC, an analytical system using supercritical fluid, was co-developed with Osaka University, Miyazaki Agricultural Research Institute, and Kobe University in a project of the Japan Science and Technology Agency. Since its release in January 2015, it has been recognised worldwide as an innovative system. The Nexera UC is now widely used in government agencies, universities and environmental testing laboratories, in addition to pharmaceutical and food industries.
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Expanded Portfolio for Biomolecules Purification Announced
With the increased interest in the purification of a wider variety of biomolecules, the portfolio of Kromasil 300 Å has been expanded to support these applications.
The Kromasil 300 Å line of HPLC products is now shipping with C18, C8, C4 and diC4 derivatisations for scientists and engineers exploiting selectivity and loadability for purification of API and impurity isolation. In particular, the new Kromasil 300 Å diC4 has different characteristic than a classic C4, enabling new opportunities to remove impurities from peptide and protein crudes.
For more information, visit
www.kromasil.com.
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Fully Automated Sample Clean-up System for PCB and Dioxin Analysis Launched
With DEXTech 16, LCTech launches the first fully automated sample clean-up system for PCB and dioxin analysis.
DEXTech 16 processes fully automated and unattended 16 samples within one sequence around the clock, even at the weekends. The specialist for automated sample preparation, LCTech GmbH, thus sets a new and unique standard with the first complete automation for the clean-up of food, feed, environmental, and biological samples in the PCB and dioxin analysis.
All 16 samples are quantitatively loaded one after the other via an autosampler and afterwards transferred onto the 3-column setup of the system
via the sample loop. The sample clean-up is based on the extensive expertise gained on the DEXTech product family using an acidic silica gel column, an aluminium oxide column, and a carbon column.
Default methods that are compliant to the EPA method and European regulations are stored in the system. However, an individual parameterisation is also possible. Existing, already evaluated customer methods on the systems DEXTech Plus and DEXTech Pure can be transferred to the fully automated system DEXTech 16.
HPLC Performance Maintenance Kits
Sciencix’s HPLC performance maintenance kits are equivalent to the corresponding OEM kits. The PM kits contain all the essential parts to keep your HPLC systems performing at an optimal level. Sciencix is a premier alternative provider of top brand HPLC instrumentation products.
For more information about Sciencix please visit
www.sales@
sciencix.com.
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The system can be prepared to start a sequence within only 30 minutes. The user just have to place the samples into the system, chose or modify a stored method, and insert the ready-to-use columns into the column carousel. Having that done, DEXTech 16 processes 16 samples one after the other and separates PCBs and dioxins in one fraction each so that they can subsequently be measured in one GC-MS run each.
Due to design and automated rinsing steps, cross-contamination is reliably avoided as proven by previous DEXTech systems.
A sophisticated and easy to handle software builds the core of the system and offers a comfortable operation via touch screen to the user including advantages like intelligent solvent management, permanent overview of process status, or report generation.
Further advantages are various safety features like controlled and monitored robotic operations, overpressure detection with ‘non-stop policy’, waste sensor, or password protected software.
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Expanded Column Range now Includes 1.8 µm Particles for Superior UHPLC Analyses
Raptor LC columns have been providing higher efficiencies, faster analyses, and rock-solid reliability to HPLC users for several years. And they have just evolved again. Restek has brought the benefits of Raptor columns to those who have made the leap to UHPLC instruments, yet are still looking to boost their efficiencies further. Already sold with 2.7 and 5 µm superficially porous particles (SPP), Raptor columns are now available with 1.8 µm particles specifically for use with UHPLC. Choose from four distinctive Restek phase chemistries: Biphenyl, ARC-18, C18, and FluoroPhenyl.
Raptor 1.8 µm columns are designed and QC tested for maximum lifetime under UHPLC pressures. And, because they are Raptor SPP LC columns, they offer the peak capacity, selectivity, and reproducibility that analysts have learned to depend on from Restek.
Outfit your UHPLC with evolutionary Raptor 1.8 µm columns today at
www.restek.com/raptor 44667pr@reply-direct.com
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