Figure 6 Conclusions
Liquid-Liquid Chromatography has matured into a valid science, which after almost 60 years deserves to be integrated into mainstream laboratory and process chromatography. L-LC been shown to compliment HPLC, with narrow range polarity cutting and by helping to find peaks co-eluting in HPLC. One beauty of L-LC is that the separation is based largely on defining on the polarities of targets, therefore classes of compounds can be separated which can then be optimised without sample loss. These narrow polarity range classes can finally be passed through a HPLC, assuming sample losses can be tolerated. If not, Sequential L-LC to L-LC with different solvents may be utilised. L-LC is a low-pressure technique (typically 100 to 500 psi) thus it can use lower price ancillary equipment than HPLC. L-LC usage has the potential for considerable solvent cost and timesavings.
Figure 7
Figure 9
Figure 8
Figure 10
References
[1] Leitao G.G, El-Adji S.S, Melo, W.A, Leitao, S.G, Brown, L., J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2041-2051 [2] Leitao G.G, Souza, P.A, Moraes, A.A, Brown, L, J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2053-2060 [3] Comprehensive Analytical Chemistry XXXVIII, A Berthod (Ed) (2002), Countercurrent Chromatography, Chapter 2, Berthod A, Brown L, Leitao G.G, Leitao. S.G [4] Silva J.C.T, Jham G.N, Oliveira, R.D. L, Brown, L., J of Chrom A (2007), 1151, 203-210 [5] Vidal S., Hayasaka, Y., Meudec, E., Cheyner, V., Skouroumounis, G.,
J.Agric. Food Chem. (2004), 52, 713-719 [6] Wangenaar F.I, Hochlowski, J.E, Pan, J.Y, Tu, N.P, Searle, P.A, J Chrom A, 1216 (2009) 4154 – 4160.
Microscale HPLC System Combines UHPLC Performance with MicroLC
Eksigent introduced the ExpressLC-Ultra. This new microscale HPLC system runs at column pressures up to 10,000 psi, allowing the use of sub- 2 micron particle columns. Along with a new UV detection system, the system delivers all the advantages of microscale LC including solvent savings, reduced sample requirements, and reduced frictional heating.
The new system’s CCD-based UV detector works with microfabricated flow cells to ensure ultra-high sensitivity with no band broadening. The ExpressLC-Ultra’s high UV sensitivity allows detection of impurities at levels as low as 0.05%, meeting the needs of researchers in drug discovery and many other applications. The system incorporates Eksigent’s proprietary Microfluidic Flow Control (MFC) technology to generate accurate and reproducible flow rates, from 1 to 50 microlitres per minute.
New features make the ExpressLC-Ultra as easy to use as any conventional HPLC instrument. Solvent changes are automated, and tubing and fingertight fittings ensure simple connections. The fiber-optic flow cell is easily connected and exchanged without alignment, and all user- configurable components and plumbing are accessible on the front of the instrument.
The ExpressLC-Ultra includes Eksigent’s control software with specialised diagnostic tools. The software provides tools for 21 CFR Part 11 compliance plus flexible data analysis and reporting capabilities. The Eksigent control software also includes integrated drivers for the most widely used mass spectrometry programmes including AB SCIEX Analyst, Thermo Scientific Xcalibur and Bruker Daltonics HyStar.
Circle no. 315
PITTCON NEWS
Automation and Chromatography Solutions
ANALYTICA NEWS
At Analytica 2010, HTA Srl introduced the HT800L - the new compact and versatile autosampler for highly precise injections into HPLC and UHPLC systems. HT800L has a large autonomy: it can handle up to 120 vials per run or 2 SBS plates, is easy to use and is assured by a user-friendly PC interface to program method and sequences and an additional keypad for maintenance and routine operations. HT800L can manage three different injection modes: full loop, partial loop and microlitre pick-up with unattended results (low cycle time, superior analytical performance).
Dilution, derivatisation, pH spiking and internal standard addition are available within the standard version: no extra cost required. Up to 11 different reagents are available to assure complex task and long autonomy. HT800L is available also biocompatible version. Cooling version is supported also.
HTA Srl also introduced the HT500PH - the new innovative HTA’s robot for plate handling. HT500PH is a fast and robust plate handler, equipped with 5-axis robotics (turret rotation, arm extension, arm elevation, wrist rotation and gripper). The high efficiency and reliability of HT500PH allow it to perform unattended operations in a repeatable, continuous and precise mode without errors. HT500PH is supplied with dedicated Windows based software that makes set-up and configuration very simple. Besides HT500PH is characterised by compact size and competitive pricing. HT500PH allows the connection of multiple instruments (such as microplate reader, washer, dispenser, bar code reader) allowing the integration of different laboratory steps to create a unique and automatised process. HT500PH can automate the implementation of a wide range of laboratory applications.
Circle no. 316
Chromatography Focus
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