43 Better Control and More Options in Fluorescence Microscopy


The use of microscopes in hospitals and laboratories covers a very wide spectrum of applications, from the identification of fungal mycelium in a clinic to the screening of cervical preparations for premalignant change in cytopathology, and the identification of acid alcohol-fast-bacilli (AAFB) in microbiology. Such procedures make use of different microscopy techniques including conventional brightfield (transmitted light), phase contrast, DIC and fluorescence techniques.


CoolLED Ltd is a design and manufacturing company specialising in high-performance LED technology. LEDs for fluorescence microscopy offer many benefits to the life scientist, from instant on/off to long lifetime, stability and precise control. With a wide range of wavelengths available, including white, CoolLED can provide LED excitation matched to fluorescent stains and microscope filter sets.


The new additions to the pE-Series of products mean CoolLED now offer single, dual, 3 or 4 wavelength units which are perfect for Live Cell and Clinical Screening work


As many hospitals and universities are actively-seeking to eradicate mercury-based products from their facilities, the benefit of these safe and environmentally-friendly illumination sources which do not use mercury will be appreciated


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Multi-Mode Microplate Detection Platform with Imaging Cytometer Introduced Molecular Devices®


has announced today the launch of its SpectraMax® additional applications and detection modes such as cellular imaging with the SpectraMax® and AlphaScreen® assays. The highly sensitive instrument accommodates the budget and throughput needs of both small and large laboratories alike.


The SpectraMax i3 Platform’s base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Patented user-exchangeable cartridge design expands the system’s detection capability making it highly versatile, and able to offer application options far exceeding those of standard readers. The MiniMax Imaging Cytometer module adds first of its kind cellular imaging to a multi-mode detection platform, enabling fluorescence and bright field cellular imaging. Combining cellular imaging with microplate-based applications offers new ways for scientists to compress their workflows and increase efficiency.


Using a combination of a flash lamp for spectral flexibility and light emitting diodes (LEDs) for excitation power, Molecular Devices has developed the patent-pending Spectral Fusion™ Illumination to deliver wavelength flexibility whilst maximising signal strength, ultimately increasing overall performance.


Data from the SpectraMax i3 System is captured and analysed using Molecular Devices’ proprietary, industry-leading SoftMax® Pro Software. The newly updated interface and Spectral Optimisation


Wizard simplifies the workflow and provides powerful data analysis features, eliminating the need for training on additional software. The SpectraMax i3 System is also available for use in GMP and GLP labs when used with the SoftMax®


Pro 6.3 GxP Microplate Data Compliance Software. MORE INFO. 149


i3 Multi-Mode Detection Platform. The system is available as a stand-alone reader with the option for users to upgrade to MiniMax™ Imaging Cytometer, and cartridges for Time-Resolved Fluorescence, Fluorescence Polarisation,


Efficient and Economical Gel Documentation UVP, LLC announces the release of the new GelDoc-It®


e Imager designed for fast, easy imaging of fluorescent and


colorimetric gels. The GelDoc-Ite accommodates a wide range of gel size on the integrated single UV, 2UV™ or 3UV™ transilluminator. Easily illuminate samples with a choice of transilluminator wavelengths for maximising fluorophore stain excitation. A unit with midrange 302nm UV is for viewing ethidium bromide and other stains, while the 3UV model provides built-in, user-selectable 302, 365 and 254nm excitation. An ethidium bromide emission filter is included as standard with the GelDoc-Ite, while a variety of optional emission filters are available to suit various imaging applications.


The modular design of the GelDoc-Ite enables placement of converter plates onto the UV transillumination surface, converting midrange UV to specific wavelengths for a variety of applications. The Visi-Blue™ converter plate converts the UV to 460-470nm blue light for viewing stains such as SYBR Green, SYBR Safe and GelGreen. When white light transillumination is required, the White Light converter plate can be used for viewing gels such as Coomassie Blue and Silver stain.


The GelDoc-Ite simplifies imaging with the intuitive, workflow-based VisionWorks® LS software package. Easy-to-use


one-touch buttons enable fast image acquisition, while the software effortlessly controls the scientific grade CCD camera which captures high quality, publication-ready images. Furthermore, researchers can conduct 1D analysis and generate extensive quantitative data using molecular weight, histograms, lane profile graphs and other tools. Once analysis is complete, exporting result data to Excel and other programs for documentation and publication is accomplished with software's integrated export tools.


With its compact size, the GelDoc-Ite Imager greatly reduces lab bench space requirements. The system is a true plug-and-play gel imager, requiring little to no user training. Its efficient design enables researchers to easily view gels using multiple light sources, quickly capture and save publication-quality images, and analyse lanes and bands. It is the only gel imager researchers will need for fluorescent, colorimetric and blue light imaging of a wide variety of gels.


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Launch of New Imaging Masterclass Webinar Series


Malvern Instruments’ new series of web-based Imaging Masterclasses kicked off on 26 February 2013 with an introduction to imaging for particle characterisation. The series will then continue through the year and will cover a broad range of imaging topics. Alongside this will run the laser diffraction Masterclass series which will discuss subjects including wet method development, dry method development, optical property selection and specification setting.


The opening ‘introduction to imaging’ webinar is designed to provide a basic understanding into the theory behind making particle characterisation measurements using imaging-based techniques. In line with Malvern’s established Masterclass concept, the whole series will deliver information on topics of specific interest to a broad audience and to people with varied levels of particle characterisation experience and technical expertise.


Intended to serve as a non-commercial, educational series, the new Imaging Masterclass program is the most recent in Malvern’s extensive webinar Masterclass collection. The series will be presented by Malvern’s technical and applications experts and is aimed at operators looking to get the most from their analytical techniques as well as those who simply wish to expand their understanding of the subject area.


For more information on the introductory imaging Masterclass or to register for this or other Malvern events, go to www.malvern.com/webinar.


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