Standard Solutions, Reagents and Reference Materials Cytometry Portfolio Expanded with 14 New Antibodies
Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC. The analyte specific reagents (ASRs) provide high specificity, convenience and flexibility in flow cytometry. A new cell permeabilization reagent is also being offered. The new monoclonals include human anti-hemoglobin F, which recognises a fetal hemogloblobin molecule; CD163 markers for anti- inflammatory signal and disease process research; and CD64 markers, which react with Fc gamma receptor 1 and binds human IgG1 and IgG3. Characterised, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments.
Rounding out the line is a monoclonal for MNDA (myeloid nuclear differentiation antigen). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. Intra-Cell™ concentrate for cell permeabilization is also being released.
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New Fluorescent materials! Starna has been developing new fluorescent reference materials to assist validation for fluorescent procedures and support instrument producers – please ask for more details.
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Highly Purified Chondroitinase ABC for Neural Regeneration Research
Amsbio, a specialist supplier of high purity reagents for glycobiology and regenerative medicine, has announced the launch of a new, highly purified Chondroitinase ABC. This new enzyme is supplied protease-free, carrier-free and with low- endotoxin levels for use in neuroscience and regenerative medicine research.
Chondroitin Sulphate Proteoglycans (CSPGs) are involved in the inhibition of axon regeneration after various forms of damage to the Central Nervous System, including stroke or spinal cord injury. The enzyme Chondroitinase ABC purified from Proteus vulgaris, degrades these CSPGs, and has been shown to promote functional recovery and neural regeneration.
Amsbio’s new Chondroitinase ABC is a direct replacement for the protease-free Chondroitinase ABC formerly supplied by Seikagaku Biobusiness Corporation which was discontinued by the manufacturer in 2011 as part of their broader decision to pull out of the research reagents and glycobiology biochemicals business.
See
www.amsbio.com/brochures/ChondroitinaseABC_flyer.pdf for more details on this new enzyme, including stability study and comparison with protease-free enzyme formerly available from Seikagaku Biobusiness Corporation.
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Drugs of Abuse Screening Benefits from Ready-to-Use Reference Materials Unilabs AB in Eskilstuna, Sweden, is taking advantage
of LGC Standards LoGiCal reference materials to perform clinical analyses and screen for drugs of abuse, as chemist Elin Widing (pictured) explained: “Unilabs is a privately owned clinical laboratory that analyses company healthcare and workplace samples, as well as samples from drug abuse centres. Samples are received from across Sweden and screened either qualitatively or semi-quantitatively using immunological assays, followed by quantitative LC-MSMS or GC-MS analysis for verification. Ready-to-use LoGiCal solutions are ideal for our purposes; it is so much simpler than weighing powders for in-house preparation of reference materials.”
“We evaluated LoGiCal solutions of tetrahydrocannabinol (THC), zopiclone and, including deuterium-labelled alpha-hydroxyalprazolam as an internal standard for quantitative mass spectrometry analysis. Analyses were run in parallel with assays that were using our existing reference materials, and the results were comparable. LoGiCal reference materials are very good quality and competitively priced and, since the initial product evaluation, we have also ordered tramadol and its metabolites for our laboratory. In the future, as we purchase more reference materials, we intend to use LoGiCal solutions whenever they are available.”
To learn more about the LoGiCal product range, visit
www.logical-standards.com MORE INFO. 100
Beckman Coulter Life Sciences offers an extensive line of human and non-human antibodies as well as a range of supporting reagents and immune monitoring products. The new antibodies are supplied in 1.0mL of buffer, are ready to use and facilitate flexibility in experimental design.
“Convenience and specificity are important features for scientists,” said Mario Koksch, Director of Strategy and Portfolio Management for Beckman Coulter Life Sciences. “These new antibodies are directed at important targets and will improve accuracy for researchers.”
A strategic partnership between the companies enables Beckman Coulter Life Sciences to further diversify their extensive flow cytometry reagent portfolio and Trillium Diagnostics to broaden the reach of their products in the global market.
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