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EMBL Hamburg Researchers use Multi-Detector Array SEC System for Enhanced Protein Characterisation by SAXS
Researchers at the European Molecular Biology Laboratory (EMBL) in Hamburg, Germany, have incorporated a multi-detector array size exclusion chromatography (SEC) system from Malvern Instruments (Viscotek TDA –Triple Detector Array) at the laboratory’s powerful small angle X-ray scattering (SAXS) beamline to improve the productivity and quality of detailed protein characterisation work. Use of the Viscotek TDA triple detector array system prior to analysis provides complementary biophysical data and simultaneously purifies samples for measurement. This results in more information and better quality data from each experiment. This innovative set-up is not only limited to the institute itself, but is widely accessible to structural biology researchers via BioStructX, a European Commission initiative that creates a single gateway to Europe’s leading synchrotron facilities.
SEC produces a fractionated sample for measurement, separated on the basis of hydrodynamic size. The Viscotek TDA incorporates refractive index, UV and light scattering detectors which in combination provide concentration and absolute molecular weight measurement for these eluting fractions. Integrating this system with the SAXS beamline provides it with purified samples for measurement that give enhanced signal-to-noise ratios and high quality data. In combination with the biophysical information from the TDA these data provide detailed information about the internal shape and structure of proteins that advances understanding of their behaviour. In particular SAXS data is valued for the determination and monitoring of the low resolution structures and conformational changes that proteins exhibit.
Both Malvern Instruments and EMBL Hamburg are partners in the BioStructX project which brings together facilities from across Europe to establish a state-of-the-art coordinated and multi-site infrastructure to support access for key methods in structural biology. The new integrated SEC-SAXS set-up was developed by a number of experts within EMBL, and with input from Malvern Instruments to enhance the department’s offering to BioSAXS users transnationally. The automated multi-pronged characterisation it offers has already demonstrated its significant advantages to the first users and is expected to be in considerable demand as the BioSructX project advances. Researchers interested in using the EMBL facility can find more information on the BioSAXS webpages.
MORE INFO. 41
New HPLC On-line Solution Secures the Analytical Workflow of Pharmaceutical Laboratories
High Performance Liquid Chromatography (HPLC) has been and continues to be a workhorse of the pharmaceutical laboratory. Throughout the product life cycle from Pre-Formulation to Quality Control, HPLC coupled to Dissolution and Content Uniformity/Assay testing are some of the biggest sample sources for HPLC analysis.
Sotax, a leader in automated solutions for dissolution testing and automated sample prep has developed a variety of ‘Off-line’ autosampler solutions over the decades. ‘Off-line’ samples are withdrawn from their instruments, immediately filtered and collected in a variety of formats. To further automate the analytical workflow and to avoid costly manual transfer mistakes, Sotax is now pleased to announce the newest addition to their line of autosamplers: the SAM Sample Manager.
The SAM guarantees a seamless flow from Dissolution samples to analytical results. Totally flexible and scalable, the system is available in three different capacities to connect to semi- automated dissolution systems and fully-automated dissolution systems: SAM S (small; for up to 240 samples), SAM M (medium; for up to 480 samples), and SAM L (large; for up to 720 samples). The SAM acts as a transfer platform keeping samples protected and injecting them one by one into HPLC, all without any vial transfer.
The system works with any HPLC instrument with ready signal functionality and can be integrated with a variety of different injection valves. SAM is driven by the WinSOTAXplus Advanced Dissolution Software while the LC data is processed with the existing chromatography software. SAM will take care of the samples seamlessly from start, to analytical finish.
Launch of Packed Columns for Biomolecule Separation and Purification Omnifit®
Labware by Diba is launching OmniSep™, a new standard in
pre-packed columns for biomolecule separation and purification. High quality agarose- and dextran-based media coupled with easy-to-use Omnifit®
glass columns make preparative chromatography cost- effective, efficient and repeatable.
“Having listened to the concerns of laboratory technicians, we wanted to create a solution that was affordable and simple and most importantly, frees up precious research time,” said Scott Brown, VP Sales and Marketing. “Omnifit®
Labware is a well-known and respected
laboratory brand, and we felt the time was right to extend our offering into gel-based media, to provide our customers with a complete, turnkey solution.”
“By design, OmniSep® media are proven to be commercially equivalent
to other leading soft-gel media, not ‘like’ and not ‘better than’,” noted Global Product Manager Trevor Hopkins. “Researchers should expect familiar chromatographic performance from the OmniSep™ media. Each column will be certified for consistency and repeatability.”
OmniSep™ offers columns packed with ten types of cross-linked agarose- and dextran-based media commonly used by laboratories for macromolecule separations. These soft gel media are available for a range of IEX, HIC, SEC and Affinity applications. The OmniSep™ media structure gives the adsorbent high chemical, physical and thermal stabilities, making it ideal for all stages of purification. The rigidity of the cross-linked media permits high volumetric flow rates resulting in fast separations with good resolution. OmniSep media are easy to use, and they tolerate the typical conditions of temperature, pH and chemical agents, including cleaning-in-place (CIP) reagents, routinely employed in biomolecule purification and separation processes.
OmniSep™ will be distributed worldwide exclusively through Kinesis, a leading international supplier of chromatography and liquid handling consumables and equipment worldwide.
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