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26 New Rotors for Centrifuges Introduced


Eppendorf introduces four new rotors for Centrifuges 5804/5804 R and 5810/5810 R, which are defined as the true workhorses of the lab with their renowned quality, reliability and versatility.


Two new swing-bucket rotors increase the capacity of Centrifuge 5804/5804 R to 1 liter and Centrifuge 5810/5810 R to 3 litre. The new 3 litre swing-bucket rotor (4 x 750mL) makes


the Centrifuge 5810/5810 R the smallest 3 litre centrifuge available and contains options for round and plate buckets, either of which can use new aerosol-tight Eppendorf QuickLock®


caps for one-handed operation.


Also, the new aerodynamic round bucket design allows for energy savings up to 36% (compared to rotor A-4-81).


In addition, there are two new fixed-angle rotors now available for both Centrifuges 5804/5804 R and 5810/5810 R. These rotors include aerosol-tight Eppendorf QuickLock lids, which require only a quarter turn! The new 48 x 1.5/2mL rotor (FA-45-48-11) has a max speed of 19,083 x g. Also available is a high-capacity fixed-angle rotor for 15mL conical and glass tubes (F-35-48-17), with capacity for 40 x 15mL conical tubes and max speed up to 5,050 x g.


MORE INFO. 84


New Independent Validation Study Shows Rapid Automated System is as Accurate as Manual Colony Counting


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Synbiosis is delighted to announce its ProtoCOL 3 rapid automated colony counter has been shown in an independent study to perform with the same level of accuracy as manual colony counting for enumerating 10 different types of microbial colonies on a range of agar plates.


The study, which was performed to GLP-compliant standards at Don Whitley Scientific Contract Microbiology Laboratory, compared the ProtoCOL 3 system with manual counting for enumeration of bacterial, yeast and fungal colonies on either Plate Count Agar, Columbia Blood Agar or Sabouraud Dextrose Agar plates.


These plates were surface spread or spiral plated with one of the following organisms: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Kocuria rhizophila, Enterococcus faecalis, Mannheimia haemolytica, Bacillus subtilis, Streptococcus pneumoniae, Candida albicans and Aspergillus brasiliensis. The resulting colonies were then enumerated both manually and using the ProtoCOL 3’s powerful software to produce a count.


For each plate type (spiral and spread) the comparison between ProtoCOL 3 and manual counts were analysed statistically using a t-test. The results (p = 0.105 for spiral plate data and p = 0.143 for spread plate data) did not identify significant differences between manual and the automated counting methods, for either plate type, at the 95% confidence level.


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Martin Smith at Synbiosis commented: “The microorganisms in this independent study produce colonies of differing colours, shapes and sizes and were also cultured on both translucent and opaque agars, which means some are a real challenge to count automatically. We’re delighted with the results of this study because they show that there is no significant difference between the accuracy of manual and automated counting with the ProtoCOL 3 in what are realistic evaluation situations you’d see in many microbiology laboratories.”


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Martin continued: “Being able to accurately enumerate so many types of colonies on different agars is a task very few automated colony counters can perform well and this study validates the ProtoCOL 3’s versatility for this application. Microbiologists looking to increase their throughput of plate counts can now install a ProtoCOL 3, confident that they will automatically count many different types of bacteria, fungi and yeast in a fraction of the time, while still guaranteeing the accuracy they demand from a manual count.”


MORE INFO. 85 MORE INFO. 86


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