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THC or CBD with CBC increases inflammatory modulation, suggesting a strong synergistic effect [20]. Studies in the 1970s suggested that CBC was the second most abundant cannabinoid in cannabis plants, although today that is no longer the case. This is due to selective breeding favouring plants high in THC and CBD, with varieties originating from the tropics with initially high concentrations of CBC having little resemblance to modern commercial hybrids [20].
Conclusion
The data demonstrates the utility of orthogonal methodology employed within a reversed-phase platform to determine the identity of an unknown constituent in Vermont hemp extract as CBC-A. In the case presented, the data obtained from the proposed ‘turn- key’ separation at a single pH, was not enough alone to draw unequivocal conclusions regarding the identity of the compound of interest. The data also reveals the utility of MS, PDA-UV, and a fraction collector such as the WFM-A, as important tools to distinguish isobaric constituents in C. sativa extract such as CBD-A, THC-A and CBC-A.
Acknowledgements
We would like to acknowledge Dr Christopher Hudalla at ProVerde Laboratories (Milford, MA, USA) for contribution of the raw hemp material.
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Figure 5: PDA-UV and QDa-MS spectra of (A) the unknown eluting at 2.1 mins in hemp extract and (B) CBC-A reference standard.
Figure 4: Overlay of the CBC-A reference standard before and after decarboxylation with partial conversion to the neutral form CBC. The chemical structure [16] is shown next to the respective peak.
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