Journal Highlights
Biological Applications
Tramadol Promotes Oxidative Stress, Fibrosis, Apoptosis, Ultrastructural and Biochemical Alterations in the Adrenal Cortex of Adult Male Rats with Possible Reversibility Aſter Withdrawal by AM Shalaby, AM Aboregela, MA Alabiad, and DF El Shaer, Microsc Microanal | doi:10.1017/S1431927620001397
Tramadol is a centrally acting analgesic drug used for the
management of moderate to severe pain in a variety of diseases. Long-term use of tramadol can induce endocrinopathy. We evaluated the effect of tramadol dependence on the adrenal cortex and the effect of its withdrawal. Tirty adult male rats were divided into three groups: the control group, the tramadol dependent group, and the recovery group. Tramadol induced severe histopathological changes in the adrenal cortex in the form of disturbed architecture, swollen cells, and shrunken cells with pyknotic nuclei
(Figure). Inflammatory cellular
infiltration and affected areas of variable size were also detected. A significant increase in P53 and Bax immunoreaction was detected and confirmed by RT-qPCR. Ultrastructural examination showed irregular, shrunken adrenocorticocytes with dense nuclei. Dilated sER, mitochondria with disrupted cristae, and numerous coalesced lipid droplets were also demonstrated. All changes started to return to normal aſter the withdrawal of tramadol. Tus, it was confirmed that long- term use of tramadol can induce severe adrenal changes with subsequent recovery.
A fasciculata cell with an irregular nucleus (N) with dilated perinuclear space (arrowhead), confluent lipid droplets (L), and cytoplasmic vacuoles (v). Notice dilated blood capillary (C) X2500.
2020 July •
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