Volume 28 Number 4 2020 July


Microscopy Today Annual Micrograph Competition 12 2020 Microscopy Today Micrograph Awards


Correlative Microscopy Contents


14 ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration Taewoo Kim, Eugene Serabyn, Kurt Liewer, Nathan Oborny, J. Kent Wallace, Stephanie Rider, Manuel Bedrossian, Christian Lindensmith, and Jay Nadeau


20 CLEM with Commercially Available Reagents to Facilitate Immunolocalization Leslie Cummins, Vincent Tu, Joshua Mayoral, Louis M. Weiss, Frank P. Macaluso, and Vera DesMarais


24 Simple Methods to Correlate Light and Scanning Electron Microscopy Claudia S. López, Erin Stempinski, and Jessica L. Riesterer


Fluorescence Microscopy


30 Microscope Hardware and Software Delays Cause Photo-Toxicity Alex Kiepas and Claire M. Brown


38 Challenges and Need for Developing Green Wavelength Technology in Life Science


Fluorescence Applications Kavita Aswani


Big Data Considerations About the Cover


42 Practical Guide to Storage of Large Amounts of Microscopy Data Andrey Andreev and Daniel E.S. Koo


Microscopy 101


46 Recipes for Consistent Selected Area Electron Diffraction Results: Part 3: Electron Diffraction Analysis Software Scott D. Walck


Microscopy Education


54 A Leeuwenhoek-Type Mobile Microscope for Histology Education Masao Maeda, Nobuteru Usuda, Masahiro Kokubo, Sumito Shirane, Motoaki Fukasawa, and Kuniaki Nagayama


Pioneers of Microscopy


Finalists in the 2020 Microscopy Today Micrograph Awards competition. Images are arranged as follows: Column 1 is the Published category; columns 2, 3, and 4 the Open unpublished category; and column 5 shows finalists in the Video category.


See page 12 for micrograph and submitter details.


60 Scanning Electron Microscope Pioneers Dennis McMullan (1923–2014) and Ken Smith (1928–2020): SEM, Whisky, Chocolate, and Fast Cars Peter Hawkes


Highlights from Microscopy and Microanalysis 66 Atomic-Resolution Cryo-Stem Across Continuously Variable Temperature 66 Deep Neural Network Enabled Space Group Identification in EBSD


67 Tramadol Promotes Oxidative Stress, Fibrosis, Apoptosis, Ultrastructural and Biochemical Alterations in the Adrenal Cortex of Adult Male Rats with Possible Reversibility After Withdrawal


Departments


7 Editorial 8 Carmichael’s Concise Review


62 Industry News 64 Product News 68 Crossword


70 NetNotes 78 Calendar of Meetings 81 Dear Abbe 82 Index of Advertisers


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