Figure 1: Automation of ELVIS. (a) The fluidic (SPU) with pumps and valves for sample delivery and staining. (b) Software interface.
Figure 2: ELVIS field performance. (a) DHM amplitude minimum projection in a single focal plane of a 300-frame (20 s) recording. (b) LFM maximum projection of the same FOV. Note that the organism indicated by the magenta arrow is highly apparent in LFM but almost invisible in the DHM image; the organism indicated by the blue arrowhead only appears in the DHM image. Note that each fluorescent spot represents one frame of the LFM maximum projection to show movement of the organism. (c) DHM minimum amplitude projection of 802 frames (53 s) after AO addition. (d) LFM maximum intensity projection of 802 frames with AO. (e–h) Close- up on a small eukaryote in DHM amplitude (e), phase (f), and unwrapped phase (g). Panel (h) shows the phase shift in radians as a surface plot. (i–l) A more complex eukaryote showing intracellular structures in amplitude (i), phase (j), unwrapped phase (k), and as a phase shift surface plot.
