Correlative Light and Electron Microscopy


serial sectioning, this method enables the researcher to easily use both fluorescence and immuno-gold labeling to identify an antigen of interest in the specific cell of interest without invest- ing in expensive CLEM technical equipment. Another advan- tage of the method is that using one labeling protocol reduces time and sample preparation.


Conclusions In this paper we show that it is possible to conduct CLEM


experiments without specialized CLEM hardware simply by taking advantage of commercially available materials, includ- ing a double-labeled secondary antibody (Alexa 488 and 10 nm gold) and a photo-etched cover glass.


Acknowledgments Tis work was done at the Analytical Imaging Facility of


Albert Einstein College of Medicine. Te work was supported by a NCI cancer center support grant P30CA013330 and a Shared Instrumentation Grant #1S10OD016214-01A1. Special thanks to Xheni Nishku for Adobe InDesign and Photoshop assistance.


References [1] FJ Timmermans and C Otto, Rev Sci Instrum 86(1) (2015) 011501.


[2] K Koistinen et al., EC Microbiology 4(5) (2016) 787–800. [3] V Tu et al., mBio (2020) doi: 10.1128/mBio.02699-19. [4] LM Weiss and K Kim, eds., Toxoplasma gondii: Te Model Apicomplexan. Perspectives and Methods, Academic Press, Cambridge, MA, 2007.


[5] V Tu et al., mSphere (2020) doi: 10.1128/mSphere.00100-20.


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