MicroscopyEducation


Figure 3: Images of 4 μm thick sections of monkey skeletal muscle with hematoxylin-eosin staining. (a), (b) An image taken with the L-eye mobile microscope, ×180 (a) and ×500 (b). At the lower magnification muscle striations are not resolved, but with the ×500 magnification striations are visible. (c), (d) Images taken with the educational ECLIPSE E100 microscope with 10× (c) and 40× (d) objective lenses. As with the L-eye mobile system, striations are not visible at the lower resolution. All images were collected with the Huawei P10 Plus smartphone. Scale bars represent 50 μm.


pyramidal cells with dendrites from the cell bodies are visible as a meshwork with both the L-eye mobile and ECLIPSE E100 microscopes. With the ×180 L-eye microscope all neurons in this thick section could be focused in the same image (Figure 4a). However, in the image from the ECLIPSE E100 micro- scope with a 10× objective lens, some neurons are focused, but others are out of focus (Figure 4b). Te neurons imaged in this 100 µm section of brain are located at different distances from the objective.


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Discussion Te resolution and image quality obtained by the L-eye


mobile microscope are comparable to those obtained with the ECLIPSE E100. Te resolution of the ×500 mobile microscope is sufficient to observe skeletal muscle fiber striations, which are not resolved with an educational microscope with a 10× objec- tive (Figure 3). Te quality of the mobile microscope images for histological sections is good enough for initial histology edu- cation and understanding tissue structure. Furthermore, with


www.microscopy-today.com • 2020 July


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