Green Wavelength Technology
a good fluorescence signal or image is dramatically reduced compared to other LED green gap solutions. Tis translates to higher throughput potential and more efficient sample pro- cessing for research laboratories. To successfully take advantage of these new capabilities,
the resulting illumination systems take into account all ther- mal, electrical, and optical parameters to maximize light con- version and delivery of light to the sample. Tis solution can be more efficient than other phosphor conversion techniques and was recognized with a Microscopy Today Innovation Award in 2016. With the LaserLED Hybrid Drive, investigators have a full optical spectrum available to excite common fluorescent proteins and fluorophores with one light source.
Figure 5: Comparison of excitation light power reaching the sample plane on an Olympus BX50 microscope using a 10× objective.
Power Equivalence Life science fluorescence imaging and measurements typi-
cally involve excitation of a fluorescence molecule, and col- lection of the emitted fluorescence via a detector, which can provide the user with a quantitative value for the detected emission or can translate this into a camera image. Microsco- pists typically use a shorter wavelength to excite a fluorophore and view the longer wavelength emitted fluorescence as an image. To evaluate the ability to overcome the green gap under actual imaging conditions, we tested excitation power at the point where fluorophore excitation occurs, that is, at the sam- ple plane of the microscope. Te test compared power reaching the sample using a mercury lamp, a LED source, and the Laser- LED Hybrid Drive technology. Te LaserLED Hybrid Drive provided power comparable
Figure 6: Comparison of excitation light power reaching the sample plane on a Zeiss microscope using X-Cite XYLIS LED, X-Cite 120Q mercury lamp, and a competitor’s LED as illumination sources.
to that of a mercury lamp, demonstrating that this technol- ogy can easily replace traditional lamp excitation sources and outperforms other solutions to fill the green gap (Figures 5–7). Tis provides investigators the opportunity to switch to a technology where short lamp lifetimes and bulb stock and stability are not concerns in completion of imaging and research projects.
Conclusion Microscopists have struggled with the low power of green
Figure 7: Comparison of the spectrum of mercury (blue) with phosphor LED (green) and LaserLED Hybrid Drive technology (red).
broad peak from 500–600 nm, which can then be filtered to a more specific excitation band depending on the excitation peak of the fluorophore of interest. With increased power in the 500–600 nm region, the exposure time required to obtain
2020 July •
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LEDs since they first entered the laboratory environment over a decade ago. Since then, technology has advanced dramati- cally, allowing manufacturers to develop innovative methods for delivering a solution to excite fluorophores in this trou- blesome green gap region. Te Excelitas´ LaserLED Hybrid Drive provides users with superior illumination uniformity and maximum light delivery at all wavelengths. Tis tech- nology enables investigators to easily switch from mercury, metal halide, or xenon lamps to LED technology and to enjoy the benefits of LED light sources, including long life, stability, lower running costs, and a smaller environmental footprint. Filling in the challenging green gap enables researchers, who are accustomed to traditional lamp technologies, to excite all fluorophores using LED illuminators without compromise.
References [1] Z Li et al., Int J Environ Res Public Health 15(12) (2018) 2766.
[2] T Pulli et al., Light: Science & Applications 4 (2015) e332. [3] YN Ahn et al., Scientific Reports 9(1) (2019) 16848.
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