Simple Methods to Correlate


Figure 1: Specific regions of interest of pancreatic ductal adenocarcinoma (PDAC) pointed out by pathologists can be studied at higher resolution using CLEM. The Toluidine Blue stained thick section in (A) can be matched to the SEM image montage in (B). By changing the overlaid image opacity from 100% in (C) to 50% in (D), specific cells can be identified in both modalities. Scale bars are equal to 200 μm.


Figure 2: CLEM workflows described in this manuscript. (A) FM and 2D SE-SEM, (B) FM and 2D-BSE-SEM, (C) FM and 2D-SEM in resin fluorescence preservation. The symbol in red indicates the FM step.


2020 July • www.microscopy-today.com 25


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