SKIN ELASTICITY TESTING
Pillet et al., demonstrated that similar spheroid models cultivated for a shorter time, five days, were not able to secrete fibrillar collagens[21]
.
Using AFM, we confirmed that the elastic tissue within 15-day-old spheroid microtissues formed a sufficiently extensive network to significantly contribute to dermal mechanics.
Likewise, AFM data revealed that elastin-rich areas within microtissues were associated with a significant reduction in tissue stiffness. Interestingly, microtissue stiffness was slightly higher than that of a dermis in vivo (250 kPa in spheroid microtissues at D15 vs 2-80 kPa depending on the age in vivo[22]
). Still, this
correlative study outlined the functionality of the neo-synthesised elastic fibres. With collagen I making up 80% of dermal proteins, elastic fibres should not be regarded as the only driver of tissue stiffness and elasticity[23]
. In this context, further work is required to assess the relative contribution of other ECM components to microtissue biomechanical properties.
CONCLUSION
Our 3D scaffold-free spheroid dermis microtissue appears to be a reliable in vitro model for investigating skin mechanical properties and may be useful for in vitro ageing studies or for the evaluation of anti-ageing ingredients.
For instance, the spheroid model has been used for the efficacy testing of Gattefossé’s latest age-defying ingredient, namely EleVastin
Authors Chloé Lorion, Amandine Lopez-Gaydon, Sébastien Bonnet, Boris Vogelgesang & Nicolas Bechetoille, Gattefossé
www.gattefosse.com Anna Drillat & Céline Paillier, BioMeca SAS
www.bio-meca.com
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June 2021 51
Figure 4 Immunofluorescence imaging of elastin within spheroids at D15. Each image is associated with its mechanical properties map (elastic modulus is represented in kPa). Areas rich in elastin are identified by dots and correspond to soft areas.
600 kPa
©Gattefossé
©Gattefossé
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