- target
positive staining for the Sox2, a proposed dermal stem cell marker. When cells dissociated from primary spheres were seeded back into classical cell culture dishes used for routine monolayer culture, numerous secondary spheres were formed. This indicates that once cells have formed primary spheres, they seem to retain a memory of the 3D progenitor phenotype, and preferentially re-form spheres where normally monolayer cultures would be expected to form.
Conclusion A stable culture of progenitor cells isolated from the dermal papilla could be established. Even after 11 passages, cells retained the ability to both form 3D spheres and express the stem cell marker Sox2, suggesting a stem cell phenotype. Using this culture we can now effectively evaluate the influence of cosmetic actives on dermal stem cells. A variety of evaluations may be made, including both molecular (i.e. stem cell marker expression) and phenotypic (i.e. number of spheres, proportion of complete spheres, serial passaging of 3D spheres etc). This approach will provide us with detailed insights into the behaviour and activity of dermal stem cells in the presence of cosmetic actives, thus enabling the evaluation of their ability to maintain or restore their regenerative potential in the dermis. Protection and vitalization of human dermal stem cells is the next generation of stem cell cosmetics. Active ingredients with these properties offer a deep-seated rejuvenation of the skin, resulting in restoration of firmness and wrinkle reduction. In addition, such products could also be beneficial in wound healing and the treatment of stretch marks.
Mibelle AG Biochemistry, Stand E70
Picture Copyright: ddrccl
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