BIOTECHNOLOGY 61
surgeries, the membrane can be sutured without
tearing of joints; ■ High thermo stability especially at low temperatures (e.g. can be frozen in
liquid nitrogen); ■ Long shelf-life if stored at room temperature in the dark (at least five
years); ■ Can be sterilised by γ-irradiation.
Fig. 2. Fibrous collagen in light microscopy, red: diameter measurement of the fibres, yellow: scale bar 100µm.
by immunohistological or fluorescence staining can be
performed directly on the CCC; ■ High mechanical stability (eg,
tensile strength); ■ Very good elasticity and flexibility;
■ High chemical resistance; ■ Nearly no auto-fluorescence; ■ Excellent biocompatibility in vitro and in vivo, with nearly no
induction of inflammation; ■ Biodegradable; ■ Excellent handling during
Terefore the CCC is predestined for use in cell biology,
regenerative medicine and tissue engineering, either as membrane alone or as cell seeded patch.
Investments into orientating in vitro and in vivo trials using CCC for selected medical indications clearly demonstrated the applicability in the field of regenerative medicine.
Te CCC was tested for different biomedical applications, eg,
REFERENCES:
Fig. 3. Ready-made CCC for use in 12 well cell culture plates: The discs are single packed and sterilised for convenient use.
1. Evaluation of a thin and mechanically stable collagen cell carrier. Schmidt T, Stachon S, Mack A, Rohde M, Just L. Tissue Eng Part C Methods. 2011 Dec;17(12):1161-70. 2. In vivo biocompatibility and biodegradation of a novel thin and mechanically stable collagen scaffold. Rahmanian-Schwarz A, Held M, Knoeller T, Stachon S, Schmidt T, Schaller HE, Just L. J Biomed Mater Res A. 2013 May 13. (Epub ahead of print). 3. Epicardial delivery of collagen patches with adipose-derived stem cells in rat and minipig models of chronic myocardial infarction. Araña M, Gavira JJ, Peña E, González A, Abizanda G, Cilla M, Pérez MM, Albiasu E, Aguado N, Casado M, López B, González S, Soriano M, Moreno C, Merino J, García-Verdugo JM, Díez J, Doblaré M, Pelacho B, Prosper F. Biomaterials 2014 Jan 35(1): 143-51. 4. Preparation and characterization of collagen-based ADSC-carrier sheets for cardiovascular application. Araña M, Peña E, Abizanda G, Cilla M, Ochoa I, Gavira JJ, Espinosa G, Doblaré M, Pelacho B, Prosper F. Acta Biomater. 2013 Apr;9(4):6075-83. 5. Improvement of skin quality using a new collagen scaffold in acute burns and reconstructive surgery: an in vivo evaluation of split skin graft transplantation in a rat model. Rahmanian-Schwarz A, Knoeller T, Held M, Just L, Schaller HE, Hirt B. Dermatol Surg. 2012 Aug; 38(8):1338-45.
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Hirschsprungs’s disease, wound healing, myocardial repair, etc, in small and large animal model and also long-term models.
Process chain We have established the whole process chain: starting from purchasing of raw material, followed by processing of the collagen and ending with producing the membranes. Tis enables us to develop tailor made collagen products for special applications or even to fulfil customer needs.
Additionally our team combines engineers and scientist (chemists, chemical engineers and cell biologist). In parallel we build up a network with co-operation partners that include clinicians which allows us to further develop collagen materials that fit perfectly for medical applications and the use in, eg, surgeries.
For more information ✔ at
www.scientistlive.com/eurolab
Silke Busch is Manager Biomedicals & Cell Biology, Viscofan BioEngineering, a
business unit of Naturin Viscofan GmbH, Weinheim, Germany.
www.viscofan-bioengineering.com
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