ANALYTICAL AND LABORATORY EQUIPMENT 35
Fig. 2. Calibration curve: Ethyl glucuronide (EtG).
3mL of methanol containing 6 per cent acetic acid. Te samples were evaporated to dryness at 40°C under nitrogen. Te residues were dissolved in 100µL of the mobile phase. 10µL injections were used for each analysis.
Te limits of detection and quantification for this method were determined to be 10ng/ mL and 50ng/mL, respectively for EtG/EtS. Te method was found to be linear from 50ng/ mg to 1000ng/mL (r2>0.999). Recoveries were found to be greater than 95 per cent for all the compounds. Interday and Intraday analysis of the EtG/EtS were <8 per cent and <10 per cent, respectively. Matrix effects were <6 per cent. Concentrations of EtG and EtS from the samples are shown in Table 2. Note:
Where EtG/EtS concentrations exceeded the upper limit of the curve, the samples were diluted and re-extracted according to the method (Ethanol concentrations: negative).
As the forensic community becomes more aware of EtG/ EtS as measureable metabolites, this procedure for the analysis of EtG/EtS will be of use in urine analysis as it shows the use of SPE extraction followed by LC-MS/ MS analysis to provide data on EtG/EtS in sexual assault cases.
For more information ✔ at
www.scientistlive.com/eurolab
Jeffery Hackett is Senior Forensic Toxicologist, UCT Inc, Bristol, PA, USA.
www.unitedchem.com. Albert A Elian
is Technical Leader, Massachusetts State Police Crime Laboratory, Sudbury, MA, USA.
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Fig. 3. Calibration curve: Ethyl sulphate (EtS). Table 2. Results of urine analysis.
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