ANALYTICAL AND LABORATORY EQUIPMENT 31


Fig. 2. Human Aβ40/Aβ42 recovery - Human plasma was diluted 1:20 and human CSF was diluted 1:4 with Sample Dilution Buffer (SensoLyte ELISA kit, Component C). Each sample was assayed 10 times.


that the assays are highly accurate and reproducible: internal coefficient of variation (CV) for the Aβ40 and Aβ42 assays are 1.7-3.1 per cent, and 4.18-5.76 per cent, respectively. Intra-assay CVs are computed as 1.9-5.6 per cent for Aβ40 and 5.36-6.12 per cent for Aβ42 kits.


Sensitivity of the kits is high, with a LOD of 2pg/ml for both Aβ40 and Aβ42 assays, as defined by an average of negative control reading plus three standard deviations.


Te SensoLyte anti-human β-Amyloid (1-42) Quantitative ELISA kit is perfectly suited for the screening of Aβ42 aggregation inhibitors.


Tis new assay offers very high performances even when low pico-molar quantities of Aβ peptides are studied. Tis is particularly relevant to in vivo events where Tioflavin T assay may not be sensitive enough.


Since Aβ aggregation is essential to the pathogenesis of AD, the use of this new anti-human Aβ (1-42) ELISA kit to search for compounds that interrupt aggregation and hence protect against the neurotoxicity of the Aβ42 fragment is of great interest. Tese new SensoLyte anti-human β-Amyloid (1-40)


Fig. 4. ELISA aggregation results are comparable with ThT assay and can provide better sensitivity when inhibitors are screened against low pico- molar quantities of Aβ42.


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Fig. 3. Transgenic mouse (Tg2576) brain lysates were diluted at 1:400 for 11 weeks old animals (TG-11W-1) and at 1:2,000 ratio for 19 weeks old animals (TG-19W-1). Non-transgenic (NT) mouse brain lysate was diluted at 100 fold. Samples were further serially diluted two-fold and quantified for the presence of Aβ40 or Aβ42 using ELISA assays.


and (1-42) ELISA kits combine high sensitivity to exceptional performance. Tey were optimized to give very precise Aβ40 and Aβ42 measurements.


Te Hyperphosphorylation of Tau proteins is also linked to AD. Te increased Tau protein levels in CSF indicate that brain cells begin to die. Accordingly, numerous anti-phospho-tau antibodies were developed to identify specific amino acids that


are phosphorylated in Tau


SensoLyte is a Registered Trademark of AnaSpec, Inc – AnaSpec is part of Eurogentec


For more information ✔ at www.scientistlive.com/eurolab


Véronique Mainfroid is Global Product Manager Proteomics and Benoît Wolf is Marketing Project Manager, EUROGENTEC,


Seraing, Belgium. www.eurogentec.com REFERENCES:


1. Selkoe DJ. Nature 399, A23 (1999);


2. Suh, Y-H. and F. Checler, Pharmacol. Rev. 54, 469 (2002);


3. Kang, J. et al. Nature 325, 733 (1987);


4. Seubert P et al. Nature 359:325-7 (1992);


5. Iwatsubo T et al. Neuron 13:45-53 (1993).


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