ANALYTICAL AND LABORATORY EQUIPMENT 11
Fig. 1. Olympus has expanded its range of compatible, interchangeable optical modules for the IX3 inverted microscope frame.
molecules in z direction - above and below the focal plane - the Leica SR GSD 3D uses the astigmatism effect. Astigmatism is actually an image aberration, but is created deliberately here with a cylindrical lens. Corresponding images allow determination of the z position of a detected molecule, thereby enabling 3D reconstruction.
To ensure reproducibility of results, the cylindrical lens is precisely positioned into the beam path as soon as the system is switched from 2D to 3D recording not by hand, but automatically by a single mouse click through software control.
Optimised imaging for wider applications
Imaging is at the heart of many analytical procedures and the latest microscope technologies are optimised for accuracy and ease of operation.
L’imagerie est au cœur de nombreuses procédures d’analyse et les technologies de microscopie de pointe sont optimisées pour plus de précision et un usage facile.
Die Bildgebung ist für viele Analyseprozesse von entscheidender Bedeutung und die neuesten Mikroskopietechnologien sind für mehr Genauigkeit und eine leichtere Handhabung optimiert.
S
uper-resolution is a major trend in light microscopy. Extending
its portfolio of super-resolution microscopy products, Leica has now launched the Leica SR GSD 3D, a wide-field system that not only offers 2D, but now also 3D super-resolution imaging of molecules and cellular structures.
Based on ground state depletion (GSD) or dSTORM technology, the wide-field fluorescence microscope attains resolutions
of down to 20 nm in the lateral and 50 nm in the axial direction. Te Leica SR GSD 3D also scores with its novel precision for localising single molecules, its system stability and its optical performance. Tese attributes are essential for reproducible, high- quality results in a very short time. Te benefits of the Leica SR GSD 3D are rounded off by the easy operation of the fully automated system and software, says the company.
Te new system is equipped with a 160x high-performance objective specifically developed for super-resolution microscopy. Its design is optimised for high-power laser emissions. Te extremely low autofluorescence ensures a high signal-to-noise ratio, which is ideal for single molecule detection applications.
To enable the localisation of
Te Leica SR GSD 3D has been optimised in several ways for reliable localisation results. All the optical components are apochromatically corrected to high Leica Microsystems standards. Building on this, the software enables precise calibration of the system for the localisation of molecules in x, y and z. Besides the standard calibration with gold beads, colour-specific calibration with fluorescence beads or dye molecules of the specimen is available. Te special SuMo technology (SUpressed MOtion) ensures minimum drift and maximum stability during detection.
“3D visualisation of cellular structures with nanometre resolution allows scientists in biomedical research to gain new insights,” explains Sebastian Tille, director of wide-field imaging at Leica Microsystems. “Te new Leica SR GSD 3D is an excellent tool for gaining a better understanding of molecular structures or cellular protein transport processes, for example. Experts are already confirming the outstanding performance and
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