Microsconullnullnull
nullFastnullSinulllenulland Safe Way to Prepare
Parafornullldehyde Solnullions
null nulln nulllis
nullicrnullcnullpy and nullmaging nullenternullnullenulls nullnullnullniversitynull2257 nullnullnullnullnullnullllege nulltatinullnnullnullnull7784null
eann.ellis@nullnullrldnet.att.net
nullepnulllymerinulld paranullnullrmaldehyde snulllutinullns have nulleen used in nullnull depnulllymerinulld paranullnullrmaldehyde. nulldd 1-2 pellets nullnull snulldium
null tnull inulln nullnullspecimens nullnullr electrnulln micrnullcnullpy since nullarnnullvsnully nullnull hydrnullnullde. nulllace this null asnullin the hnullnulld.
prnullpnulled the cnullmnullinatinulln nullnullglutaraldehyde and paranullnullrmaldehyde 2. nulleat the renulluired vnulllume nullnulldeinullninulld tnull er nullnullr 1 minute nulln
nullnullr imprnullved specimen preservatinulln. nullreparatinulln nullnulldepnulllymerinulld
high in the micrnullvnull e.
paranullnullrmaldehyde snulllutinullns renulluires the use nullnullheat (apprnullnullmately
null nulln the hnullnulldnull add the heated tnull er tnull the null asnull nullnull
60null) and raising the pnull nullt null lanull place a heating stir plate in the
paranullnullrmaldehyde and snulldium hydrnullnullde pellets. nullnullirl the null asunnull til
hnullnulld and then heat t60null null nullnullllnullnulled nully adding drnullps nullcnullnull ncentrated
the snulllutinulln clearsnullnullhich usually tanulles 1-2 minutes.
snulldium hydrnullnullde snulllutinulln until the snulllutinulln nullnullparanullnullrmaldehyde
4. null e snulllutinulln nullnull depnulllymerinulld paranullnullrmaldehyde is nnullnull
clears. nullprecise temperature nullnull60null is nnullt renulluirednullraining the
ready nullnullr use in preparing the null tnull ive.
pnullnully adding snulldium hydrnullnullde is the mt null impnullrtant actinulln in
null e tnulltal time nullnullr this preparatinulln is nnullmnullre than 5 minutesnulla
achieving depnulllymerinulltinulln and clearing nullnullthe paranullnullrmaldehyde
time savings nullnullanullnullut null minutes. nulln additinullnnullthere is nnullneed nullnullr a
snulllutinulln.
hnullt plate.
null e nullnullllnullnulling time-saving and sanulle prnulltnullcnulll has nulleen used in
my lanullnullnullr a numnuller nullnullyearsnull nullferences
1. nulleigh nullut the renulluired amnullunt nullnull paranullnullrmaldehydenull nullnullnull nullarnnullvsnullynullnullnullell nullinulll 27 (1965) 1nullnull
and put it intnullan apprnullpriate sinull null asnulltnullhnullld the null nal snulllutinulln
48
doinullnull.nullnullnullnullnull929null999nullnull
www.microscopy-today.com null nullnull nullvenuller
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