RESULTS: TITAN PROTECTED PROBIOTIC YEAST SURVIVES THE PELLETING PROCESS Log CFU/g of feed
Expected count log CFU/g
TITAN
Beadlet A Beadlet B Beadlet C
7 +/- 0.5 log 7 +/- 0.5 log 7 +/- 0.5 log 7 +/- 0.5 log
vs. expected at 65° +0.15 -0.33 -2.68 +0.43
Table 1: Pelleting stability of different yeast sources & technology at 65°C and 75°. CFU/G
1,00E+08 1,00E+07 1,00E+06 1,00E+05 1,00E+04 1,00E+03 1,00E+02
-0.57 log -1.69 log -3.48 log TITAN ‘s unique coating matrix
Fig. 2: Pelleting stability of different yeast sources & technology (85°C). TITAN
Beadlet A Beadlet B Beadlet C
RESULTS: TITAN PROBIOTIC YEAST REMAINS STABLE IN FEED FOR AT LEAST 3 MONTHS Stability is also about feed storage conditions and shelf life. Once probiotics are included in the feed, in order to be active and efficient in the animal, they must survive throughout the storage duration at the feedmill and/or on farm.
CFU/G
1,00E+08 1,00E+07 1,00E+06 1,00E+05 1,00E+04 1,00E+03
T0 65°C T1 month T2 month T3 month
Fig. 3: Stability of TITAN vs. bead technology in pelleted feed over 3 month (65°C). Measured vs.
Expected count log CFU/g
TITAN (T1 month) TITAN (T3 month)
Beadlet A (T1 month) Beadlet B (T1 month) Beadlet C (T1 month)
7 +/- 0.5 log 7 +/- 0.5 log 7 +/- 0.5 log 7 +/- 0.5 log 7 +/- 0.5 log
expected at 75°C +0.21 +0.06 -1.51 -2.30 -0.55
Looking at stability results over of the batch pelleted under the less challenging conditions (65°C), we observe that:
• At T0, products all still within the acceptable tolerance range. • At T1 month, only TITAN and beadlet C remained stable. • At T2 month, only TITAN is stable.
Log CFU/g of feed vs. expected at 75°
+0.19 -0.79 -2.74 +0.10
At 65°C, TITAN and two non-coated yeast sources are at the expected count. At 75°C, only one non-coated yeast source out of TITAN still resists, but we start to observe a drop in CFU. TITAN remains perfectly stable at both temperatures.
At 85°C, simple bead technologies (non-coated) do not survive and show a significant drop of CFU. TITAN is the only product remaining perfectly stable throughout these conditions of the feed manufacturing process.
TITAN
Beadlet A
Beadlet B
Beadlet C Measured vs.
expected at 85°C +0.12 -0.03 -1.52 -2.30 -1.36
Table 2: Stability of TITAN vs. bead technology in pelleted feed over the storage time (75°C and 85°C).
DISCUSSION: Even though yeasts with non-coated bead technology are sometimes marketed as “stable and alive” after pelleting, it seems that the yeast cells can be strongly damaged and can die during standard storage duration.
CONCLUSION:
When selecting a probiotic yeast it is crucial to ensure the live yeast will: survive feed or premix processing; tolerate the combination of other ingredients; and endure storage prior to reaching the animal. Is your live yeast provider willing to offer feed analysis to ensure the recovery of probiotic cells at the declared CFU level on your feed ticket?
GERMANY IFF FEED COMPOUNDER JULY/AUGUST 2019 PAGE 29
At 75 and 85°C, the results are even more clear: only TITAN remains stable after one month.
After 3 months of storage, TITAN still reaches the guaranteed level of CFU/g.
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