Non-Hardening Mountant Solutions Glycerol-Based


Citifluor AF1 Mountant Solution (continued)


Right: Graph showing how the viscosity of AF1 solution is influenced by adding AF3 solution


Other Useful Advice


(a) If there is some initial quenching of fluorescence:


In some cases, the reduction in the rate of PHOTOBLEACHING may be accompanied by a reduction in the initial intensity of the fluorescence signal. By diluting the AF1 solution with glycerol the reduction in the intensity of the fluorescence signal can oftentimes be mitigated.


Dilution of AF1 with glycerol will increase its refractive index whereas dilution with water will reduce its refractive index.


(b) Use as a hardening mountant:


To a poly (vinyl alcohol), e.g. Airvol 203 (Air products) or Mowiol®


4-88 (Calbiochem) solution (20% in


water), add ~20% by volume of AF1. This solution is best used soon after it is prepared as it doesn't have a long shelf-life.


Cat No.


RT RT


17970-25 17970-100


Description Citifluor AF1 Citifluor AF1


Qty. 25 ml 100 ml


n Citifluor AF1 plus DAPI Mountant Solution Usage


AF1 is a mountant solution composed of glycerol, phosphate buffered saline an antifadent to which DAPI has been added at a concentration of 2 µg per ml. If a lower concentration of DAPI is required, dilution should be carried out by adding a further quantity of AF1 solution. The solution will be of value to those using the FISH technique since the DAPI will stain the nuclei and the antifadent will prevent its bleaching.


The solution has a pH of ~10. The solution should be pipetted onto the specimen and then a cover slip applied. If the slides are stored in a refrigerator, the viscosity of the mountant solution increases thereby helping to keep the cover slip in place. There is no need to seal the cover slip with nail varnish.


Specimens mounted in AF1 solutions have been kept in this way for many months without suffering damage.


Properties and Storage


The solution is of medium viscosity and has a water- white in appearance. It may be stored at room temperature and ideally between 50° and 150° and out of strong sunlight. The cap of the bottle or if using the pipette supplied with the material, the cap which covers the pipette delivery point, should always be replaced after use since the solution is hygroscopic. Samples stored under these conditions for 6 months have shown no apparent deterioration.


Cat No. RT 17970-125 Description Qty. Citifluor AF1 + DAPI 15 ml


Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email: sgkcck@aol.com or stacie@ems-secure.com • www.emsdiasum.com


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