Non-Hardening Mountant Solutions Glycerol-Free Solutions


n Citifluor CFPVOH Plus AF100 Mountant Solution Usage


CFPVOH plus antifadent is an aqueous solution of poly (vinyl alcohol) containing an antifadent and is intended for use where it is desired to have a permanent mountant for a specimen which has been fluorescently labeled. The antifadent helps retard bleaching of fluorescent label with the extent of retardation being very dependent upon the type of label and the molecular structure of the specimen.


A few drops of the solution should be applied to the specimen followed by a cover slip. The water evaporates to give a clear film which holds the cover slip in place, thereby aiding the safe storage of the specimen.


If bleaching of the label remains unacceptably high, try a mixture of CFPVOH plus antifadent with AF100 mixed in the ratio of 10:1 (v/v). This solution will have a short shelf life (a few days).


Properties and Storage


The solutions are of medium viscosity, are water-white in appearance and have a pH of ~8.5. They contain a small amount of sodium azide to prevent fungal growth. They may be stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water, which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 2 months have been found to exhibit little apparent deterioration, e.g. no gel formation.


Cat No. RT 17978-35 Description


Citifluor CFPVOH + AF100*


* Only available when ordering with CFPVOH n


Citifluor AF87 Mountant Solution High Refractive Index


Usage


AF87 is a non-fluorescent immersion oil of medium viscosity and has a refractive index of 1.516 and may be used as an immersion oil or as a mountant medium.


If used as an immersion oil it is recommended that the microscope objective is wiped clean after use. A useful solvent mixture for removing the immersion oil is composed of ether / ethanol (7:3 v/v) or alternatively, xylene may be used.


To use AF87 as a mountant medium it is necessary to dehydrate the sample prior to application by washing it with successive amounts of absolute ethanol prior to drying. Once the sample is dry the AF87 may be applied. The AF87 contains an antifadent to reduce the amount of fluorochrome fading. AF87 has been found useful for examining specimens generated using the technique of FISH, labeled with the fluorochromes DAPI and Cy dyes.


Properties and Storage


The immersion oil may be stored at 5° to 100°C and should be kept out of strong sunlight. It should NOT BE STORED IN A REFRIGERATOR. Occasionally small crystals are formed in the oil, but careful use of a pipette can obviate the crystals interfering with the function of the oil.


Cat No.


RT RT


17976-10 17976-25


Description


Citifluor AF87 Citifluor AF87


Qty.


10 ml 25 ml


Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email: sgkcck@aol.com or stacie@ems-secure.com • www.emsdiasum.com


15 Qty. 25 ml + 5 ml


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