Non-Hardening Mountant Solutions Glycerol-Based, High Refractive Index Solutions


n Citifluor CFM-1 Mountant Solution Usage


This glycerol-phosphate buffered saline based solution has been specially formulated so as to have a refractive index of ~1.52 (at room temperature). This refractive index will be a close match to that of the glass of the objective lens and cover-slip. The CFM-1 solution will be particularly useful for high magnification work where immersion oils are use to minimise distortion of the image due to refraction of the viewing light. The solutions should also be particularly valuable where three-dimensional imaging of specimens is carried out using confocal microscopy.


A few drops of the solution should be applied to the specimen followed by a cover slip. An immersion oil (which may be CFM solution if its viscosity is appropriate) is applied in the usual way.


Properties and Storage


The solutions are of medium viscosity, are water-white in appearance and have a pH of ~7.5. They may be


n


Citifluor CFM-1 Plus Antifadent Mountant Solution


Usage


This glycerol-phosphate buffered saline based solutions has been specially formulated so as to have a refractive index of ~1.52 (at room temperature) and contains an antifadent to retard the bleaching of fluorochromes. The refractive index of the solution is designed to match the glass of the objective lens and coverslip. The CFM 1 Solution Plus Antifadent will be particularly useful for high magnification work where immersion oils are used to minimise distortion of the image due to refraction of the viewing light and where bleaching of the fluorochrome occurs. The solution should also be very valuable for three-dimensional imaging of specimens using confocal fluorescence microscopy where integrity of the image has to be maintained. To obtain images at some depth within the tissue it may be necessary to reduce the refractive index of the supplied solution. This may be done by adding sufficient glycerol to render clarity.


A few drops of the solution should be applied to the specimen followed by a cover slip.


RT RT


stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration.


Cat No.


17980-10 17980-25


Description Qty.


Citifluor CFM-1 10 ml Citifluor CFM-1 25 ml


Properties and Storage


The solution is of medium viscosity, has a water-white in appearance and a pH of ~9 The solution should be stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration.


Cat No. RT RT


17981-10 17981-25


Description


Citifluor CFM-1 + Antifadent


Citifluor CFM-1 + Antifadent


Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email: sgkcck@aol.com or stacie@ems-secure.com • www.emsdiasum.com


Qty.


10 ml 25 ml 11


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