Hardening Mountant Solutions Glycerol-Based, Film-Forming Polymer Solutions n Citifluor Tris-MWL 4-88 Mountant Solution


Usage Tris-MWL 4-88 solution is a solution of poly (vinyl alcohol), Mowiol®


4-88, in a


water/glycerol/tris buffer mix and has a pH of 8.5. Following loss of water it forms good clear films and is therefore used as a permanent mountant. A few drops of the solution should be applied to the specimen followed by a cover slip. The water evaporates to give a clear film which holds the cover slip in place, thereby aiding the safe storage of the specimen.


Properties and Storage


The solutions are of medium viscosity, are water-white in appearance and have a pH of ~8.5. They may be stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water, which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration, e.g. no gel formation.


If you want a solution of higher viscosity, we can make these to order.


If bleaching of the fluorescent marker is a problem, you may add AF100 as you would to CFPVOH (i.e. 9 parts MWL solution plus 1 part AF100). These mixtures have a limited shelf-life.


Cat No. RT 17977-150 Description Citifluor MWL4-88 Qty. 25 ml


n Citifluor CFPVOH and AF100 Mountant Solutions Usage


CFPVOH plus antifadent is an aqueous solution of poly (vinyl alcohol) containing an antifadent and is intended for use where it is desired to have a permanent mountant for a specimen which has been fluorescently labeled. The antifadent helps retard bleaching of fluorescent label with the extent of retardation being very dependent upon the type of label and the molecular structure of the specimen.


A few drops of the solution should be applied to the specimen followed by a cover slip. The water evaporates to give a clear film which holds the cover slip in place, thereby aiding the safe storage of the specimen.


If bleaching of the label remains unacceptably high, try a mixture of CFPVOH plus antifadent with AF100 mixed in the ratio of 10:1 (v/v). This solution will have a short shelf life (a few days).


Properties and Storage


The solutions are of medium viscosity, are water-white in appearance and have a pH of ~8.5. They contain a small amount of sodium azide to prevent fungal growth. They may be stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water, which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 2 months have been found to exhibit little apparent deterioration, e.g. no gel formation.


Cat No. RT 17978-35 Description


Citifluor CFPVOH + AF100*


* Only available when ordering with CFPVOH


Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email: sgkcck@aol.com or stacie@ems-secure.com • www.emsdiasum.com


17 Qty. 25 ml + 5 ml


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